GM-CSF, human, recombinant (E. coli)

Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor (E. coli-derived).

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GM-CSF, human, recombinant (E. coli)
C-60424
  20 µg
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Recombinant Human Granulocyte and Macrophage Colony Stimulating Factor (rHu GM-CSF) is a potent species specific stimulator of bone marrow cells. rHu GM-CSF also stimulates precursor
cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14.48 kDa protein containing 127 amino acid residues.

Molecular Mass 14.5 kDa
Source E. coli
Specific Activity ≥ 1 x 10⁷ IU/mg
Biological Activity The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is F 0.1 ng/ml, corresponding to a specific activity of B 1 x 10⁷ units/mg (WHO standard 88/646, NIBSC).
Formulation Lyophilized from a sterile protein solution extensively dialyzed against 2 mM sodium phosphate buffer (pH 7.4).
Reconstitution Please Note: Always centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1 – 1.0 mg/ml. For further dilution carrier protein should be added to avoid loss of bioactivity.
Purity Greater than 98% by HPLC and SDS-PAGE analysis.
Endotoxin Level < 0.1 ng per µg of rHu-CSF (1EU/µg)
Stability & Storage The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rHu GM-CSF should be stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.