GM-CSF, human, recombinant (E. coli)
Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor (E. coli-derived).
Recombinant Human Granulocyte and Macrophage Colony Stimulating Factor (rHu GM-CSF) is a potent species specific stimulator of bone marrow cells. rHu GM-CSF also stimulates precursor
cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14.48 kDa protein containing 127 amino acid residues.
|Molecular Mass||14.5 kDa|
|Specific Activity||≥ 1 x 10⁷ IU/mg|
|Biological Activity||The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is F 0.1 ng/ml, corresponding to a specific activity of B 1 x 10⁷ units/mg (WHO standard 88/646, NIBSC).|
|Formulation||Lyophilized from a sterile protein solution extensively dialyzed against 2 mM sodium phosphate buffer (pH 7.4).|
|Reconstitution||Please Note: Always centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1 – 1.0 mg/ml. For further dilution carrier protein should be added to avoid loss of bioactivity.|
|Purity||Greater than 98% by HPLC and SDS-PAGE analysis.|
|Endotoxin Level||< 0.1 ng per µg of rHu-CSF (1EU/µg)|
|Stability & Storage||The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rHu GM-CSF should be stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.|