Follicle Dermal Papilla Cell Growth Medium
Low-serum cell culture medium for dermal papilla cells from fetal, neonatal, and adult tissue.
PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells.
The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of follicle dermal papilla cells.
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
|Media supplement||Final supplement concentration|
|Fetal Calf Serum||0.04 ml / ml|
|Bovine Pituitary Extract||0.004 ml / ml|
|Basic Fibroblast Growth Factor (recombinant human)||1 ng / ml|
|Insulin (recombinant human)||5 μg / ml|
Technical Library (2)
What is the source of PromoCell’s Normal Human Follicle Dermal Papilla Cells? For how long can they be maintained in culture?
HFDPC are isolated from the hair papilla of normal human scalp hair follicles. Hair papilla in the adult hair follicle play a crucial role in the dermal-epidermal interactions that control hair production and in hair growth cycle events. The follicle dermal cells are cryopreserved at second passage and can be cultured for at least 10 population doublings when using PromoCell Follicle Dermal Papilla Cell Growth Medium (Cat. C-26501). Typical population doubling times are between 20-36 hrs.
The recommended seeding density after thawing/trypsinization is 5,000-10,000 cells/cm2. Using 1:4 splits, you can perform 4-5 passages with the cells.
Related Links and Documents
Can I use the PromoCell Growth Media for primary cells of other species?
The PromoCell specialized media are optimized for in vitro-culture of human cells. In most cases, they can also be used for cells from several other species (eg. rat, murine, bovine, porcine cells) and etablished cell lines.
Please see the attached document for references that have used our media for other cell types and species.
Related Links and Documents
- What is the source of PromoCell’s Normal Human Follicle Dermal Papilla Cells? For how long can they be maintained in culture?
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Reference Literature (22)
Effect of sinapic acid on hair growth promoting in human hair follicle dermal papilla cells via Akt activation
Woo et al.; Arch Dermatol Res. 2017 Jul;309(5):381-388
Hair growth-promoting effect of human dermal stem/progenitor cell-derived conditioned medium
Shim et al.; Tissue Engineering and Regenerative Medicine. 2015 Aug;12(4):268-275
Emblica (Phyllanthus emblica Linn.) fruit extract promotes proliferation in dermal papilla cells of human hair follicle
Luanpitpong et al.; Res. J. Med. Plant. 2011 5: 95-100
Glycyrrhizic acid attenuates stem cell-like phenotypes of human dermal papilla cells
Kiratipaiboon et al.; Phytomedicine. 2015 Dec 15;22(14):1269-78
Gene expression in hair follicle dermal papilla cells after treatment with stanozolol
Reiter et al.; Biomark Insights. 2009;4:1-8
PDGF-AA-induced filamentous mitochondria benefit dermal papilla cells in cellular migration
Mifude and Kaseda; Int J Cosmet Sci. 2015 Jun;37(3):266-71
Ciprofloxacin improves the stemness of human dermal papilla cells
Kiratipaiboon et al.; Stem Cells International. 2015; Article ID 636513
Hydroxyl radical mediates cisplatin-induced apoptosis in human hair follicle dermal papilla cells and keratinocytes through Bcl-2-dependent mechanism
Luanpitpong et al.; Apoptosis. 2011 Aug;16(8):769-82
Malva verticillata seed extracts upregulate the Wnt pathway in human dermal papilla cells
Lee et al.; Int J Cosmet Sci. 2016 Apr;38(2):148-54
Hair-growth-promoting effect of conditioned medium of high integrin alpha6 and low CD 71 (alpha6bri/CD71dim) positive keratinocyte cells
Won et al.; Int J Mol Sci. 2015 Feb 19;16(3):4379-91
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