Glutathione S-transferase (GST) is an essential enzyme involved in detoxification of xenobiotics mediating conjugation of glutathione with those compounds.
The GST Activity Assays can detect GST activity in crude cell lysate or purified protein fractions and the kit is also suited for quantitation of GST-tagged fusion proteins. They are based upon the GST-catalyzed reaction between GSH and the GST substrate MCB (Fluorometric Kit).
The free form of MCB is almost non-fluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when reacting with glutathione. GST catalyzes the MCB-glutathione reaction, and the fluorescence levels are proportional to the amount of GST present in the reaction. Therefore, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader.