Fluorometric Cell Viability Kit II (Calcein)

Fluorometric cell viability and cytotoxicity detection.

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Fluorometric Cell Viability Kit II (Calcein)
  1.000 assays
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The PromoKine Fluorometric Cell Viability Kit II is intented for the reliable fluorometric determination of viable cell numbers. It utilizes the cell membrane-permeable, non-fluorescent dye Calcein-AM that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein. Since esterases are only active in viable cells, the amount of Calcein-AM hydrolyzed by cellular esterases is directly proportional to the number of viable cells in culture.

Figure 1. Assay mechanism of Calcein_AM conversion by intracellular esterases.