Fluorometric Cell Viability Kit II (Calcein)
Fluorometric cell viability and cytotoxicity detection.
The PromoKine Fluorometric Cell Viability Kit II is intented for the reliable fluorometric determination of viable cell numbers. It utilizes the cell membrane-permeable, non-fluorescent dye Calcein-AM that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein. Since esterases are only active in viable cells, the amount of Calcein-AM hydrolyzed by cellular esterases is directly proportional to the number of viable cells in culture.