In PromoCell’s Enolase-α Inhibitor Screening Kit, Enolase-α catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed probe generating a colorimetric signal (OD 570 nm). In the presence of Enolase-α inhibitor, the reaction is impeded. An Enolase-α Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is simple, fast, high-throughput adaptable and can be completed in less than 1 hour.
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