PromoCell Endothelial Cell Growth Medium MV2 was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.
Endothelial Cell Growth Medium MV2 lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains the basic Fibroblast Growth Factor, the Insulin-like Growth Factor (Long R3 IGF-1) and the Vascular Endothelial Growth Factor (VEGF). Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on the cell metabolism, it may also interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.
To view a list of references where this medium was used with other species/cell types please click here.
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
|Media supplement||Final supplement concentration|
|Fetal Calf Serum||0.05 ml / ml|
|Epidermal Growth Factor (recombinant human)||5 ng / ml|
|Basic Fibroblast Growth Factor (recombinant human)||10 ng / ml|
|Insulin-like Growth Factor (Long R3 IGF)||20 ng / ml|
|Vascular Endothelial Growth Factor 165 (recombinant human)||0.5 ng / ml|
|Ascorbic Acid||1 μg / ml|
|Hydrocortisone||0.2 μg / ml|