DNA Fragmentation Detection Kit II (BrdU)

TUNEL-based assay kit for sensitive, red-fluorescent detection of DNA fragmentation in cultured cells and tissue sections.

DNA Fragmentation Detection Kit II (BrdU)
  60 assays

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DNA Fragmentation Detection Kit II (BrdU)

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PromoCell’s DNA Fragmentation Detection Kit II (BrdU) provides all ready-to-use components including positive and negative control cells for convenient detection of DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g. fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a red fluorescent-labeled anti-BrdU monoclonal antibody. The assay is also suitable for studying apoptosis with GFP transfected cells.