The PromoCell DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells.
The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.
PromoCell DetachKit was designed for the safe and efficient detachment of primary human cells in routine subculturing. Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%.
HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.