Colorimetric Cell Viability Kit I (WST-8)

Colorimetric cell viability and cytotoxicity detection

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The Colorimetric Cell Viability Kits I allows for easy and reliable colorimetric determination of viable cell numbers with excellent sensitivity and linearity. The kit utilizes the tetrazolium salt WST-8 that is reduced to water-soluble, orange formazan dyes by dehydrogenases present in viable cells. The absorbance of the formazan dye is proportional to the number of metabolically active cells and can be measured directly from a 96-well plate without additional processing.

Figure 1. Figure: Assay mechanism of WST-8 conversion by dehydrogenases in viable cells. (Click to enlarge image).