Cell Proliferation/Cytotoxicity Kit
Highly sensitive, fluorometric assay detecting as less as 30 proliferating or necrotic cells.
PromoCell’s Cell Proliferation/Cytotoxicity Kit (fluorometric) provides an easy, rapid and quantitative method to measure cell proliferation as well as cytotoxicity. The assay is based on a nuclear dye that specifically binds to nucleic acid in the cell nucleus and emits green fluorescence. The generated fluorescent intensity is directly proportional to the cell number, which can be quantified by measuring fluorescence at Ex/Em = 480/538 nm using a fluorometer or fluorescence plate reader. This assay kit provides a just add-and-read, non-radioactive, and high-throughput method that is more sensitive than MTT, XTT, or MTS-based assays, and can detect a wide linear range of 25-60,000 cells