Caspase-7 Immunoassay Kit
Specifically detect Caspase-7 activity in microtiter plate.
Caspases play a key role in apoptosis. The PromoKine Caspase-7 Immunoassay Kit provides an effective immunosorbent enzyme assay for the specific, quantitative detection of Caspase-7 activity in microtiter plates. The assay utilizes Caspase-7 polyclonal antibody to capture activated Caspase-7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated Caspase-7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase-7. Other known caspases and non-specific proteases are not detected.