Caspase-3 Immunoassay Kit
Specifically detect Caspase-3 activity in microtiter plate.
Activation of Caspase-3 plays a key role in initiation of cellular events during apoptosis. The PromoKine Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of Caspase-3 activity in microtiter plates. The assay utilizes Caspase-3 polyclonal antibody to capture activated Caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated Caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase-3. Other known caspases and non-specific proteases are not detected.