Cancer Cell Line Medium XF

Xeno-free medium for adherent culture of cancer cell lines.

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Cancer Cell Line Medium XF
C-28077
  250 ml
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The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:
The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).

Key features:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

Tissue Tested Cell Lines Cell Lines Origin Recommended Coating
Brain BV2 immortalized murine primary microglial cells Fibronectin
1 µg/cm2
Breast MCF-7 pleural effusion of metastatic human breast adenocarcinoma Fibronectin
1 µg/cm2
Colon HT-29 human colon adenocarcinoma Vitronectin
0.5 µg/cm2
Connective tissue HT1080 human fibrosarcoma Fibronectin
1 µg/cm2
Liver HepG2 hepatocellular carcinoma of the human liver Vitronectin
0.5 µg/cm2
Lung A-549 human lung carcinoma Vitronectin
0.5 µg/cm2

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