Cancer Cell Line Medium XF

Xeno-free medium for the standardized in vitro cultivation of established cancer cell lines.

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Cancer Cell Line Medium XF
C-28077
  250 ml
$129.00

The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:
The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).

Key features:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

Tissue Tested Cell Lines Cell Lines Origin Remarks
Brain BV2 immortalized murine primary microglial cells Coat with Fibronectin:1 µg/cm2
Breast MCF-7 pleural effusion of metastatic human breast adenocarcinoma Coat with Fibronectin:1 µg/cm2
Colon HT-29 human colon adenocarcinoma Coat with Vitronectin:0.5 µg/cm2
Connective tissue HT1080 human fibrosarcoma Coat with Fibronectin:1 µg/cm2
Liver HepG2 hepatocellular carcinoma of the human liver Coat with Vitronectin:0.5 µg/cm2
Lung A-549 human lung carcinoma Coat with Vitronectin:0.5 µg/cm2
Prostate LNCaP lymph node metastasis of human prostate adenocarcinoma 3D culture in C-28070 is recommended
Peripheral blood MV-4-11 Human acute myelogenous leukemia (suspension) No coating required
Bone marrow KG-1 Human acute myelogenous leukemia (suspension) No coating required
Kidney ACHN Human Renal Cell Carcinoma Coat with Fibronectin:1 µg/cm2
Brain A172 Human Glioblastoma Coat with Fibronectin:1 µg/cm2
Skeletal muscle C2C12 Mouse Myoblasts Coat with Fibronectin:1 µg/cm2
Skin B16-F10 Mouse Melanoma Coat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumor RAW264.7 Mouse Macrophage Coat with Fibronectin:1 µg/cm2
Brain, neuroectodermal Neuro-2a Mouse Neuroblastoma Coat with Fibronectin:1 µg/cm2
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.

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