Cancer Cell Line Medium XF
Xeno-free medium for the standardized in vitro cultivation of established cancer cell lines.
The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:
|Tissue||Tested Cell Lines||Cell Lines Origin||Remarks|
|Brain||BV2||immortalized murine primary microglial cells||Coat with Fibronectin:|
|Breast||MCF-7||pleural effusion of metastatic human breast adenocarcinoma||Coat with Fibronectin:|
|Colon||HT-29||human colon adenocarcinoma||Coat with Vitronectin:|
|Connective tissue||HT1080||human fibrosarcoma||Coat with Fibronectin:|
|Liver||HepG2||hepatocellular carcinoma of the human liver||Coat with Vitronectin:|
|Lung||A-549||human lung carcinoma||Coat with Vitronectin:|
|Prostate||LNCaP||lymph node metastasis of human prostate adenocarcinoma||3D culture in C-28070 is recommended|
Technical Library (4)
Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
During our quality control we do not determine the presence or maintenance of respective stem cell markers in the tumorspheres. We use a functional approach instead, by culturing the tumorspheres over serial passages in C-28070. Tumorsphere formation requires the biological features of Anoikis resistance and self-renewal and therefore indicates the presence of cancer stem cells (CSC)/cancer initiating cells (CIC) in the spheres. 3D tumorsphere culture thus allows scientists to study the biology of CSCs without any background knowledge on CSC markers.
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Can I use the 3D Tumorsphere Medium XF for mouse cell lines?
Yes, we have received a customer feedback that our 3D Tumorsphere Medium XF (C-28070) has been successfully used for tumorsphere formation of mouse cell lines.
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I would like to know the number of population doublings for PromoCell mononuclear cells (C-12907/C-12901) when grown in Mononuclear Cell Medium (C-28030).
Mononuclear cells are mostly used in immunology, infection biology, hematology and cancer research to study subpopulations of blood cells.
Our Mononuclear Cell Medium (C-28030) is intended for short-term maintenance (up to 48 hrs) of the thawed hMNC before you proceed with your experiments. The number of PDs will depend on the subsequent cell culture conditions and is not determined by PromoCell.
Please note: Depending on the conditions, the ratio of the subpopulations will gradually change, as the different blood cell types behave in different manners. Researchers normally start soon after thawing to either select the cell type of their interest (e.g. hematopoietic cells, endothelial progenitor cells) or perform experiments with all populations of hMNCs (e.g. to study effects on toxicity, viability or metabolism).
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What are the criteria for selection or exclusion of peripheral blood donors? Is the peripheral blood that PromoCell use to isolate blood cells (MNC-PB, monocytes) derived from completely healthy donors?
The following criteria are applied for blood donations:
a) Donors with light infection like a cold or cough are excluded for one week b) Donors with infections with a temperature above 37.9°C and/ or antibiotics therapy are excluded for 4 weeks c) Blood pressure: exclusion only if systolic blood pressure is < 100 mm Hg or > 180 mm Hg or if diastolic blood pressure is > 100 mm Hg. No exclusion if blood pressure is drug treated and in acceptable range d) High cholesterol: no exclusion even though medication is used e) Diabetes: exclusion if diabetes type I or use of insulin f) Steroid use: exclusion for 4 weeks after application g) Cancer: exclusion h) Other chronic diseases: exclusion depends on the type of disease (for example chronic heart disease, autoimmune disease)
Related Links and Documents
- Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
Application Notes (6)
Selective In Vitro Culture of Primary Human Cancer Cells from Human Tumor SamplesIsolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the Primary Cancer Culture System and how it compares to other established culture methods.
Standardized Culture of Established Cancer Cell Lines under Serum-free and Xeno-free ConditionsUndefined media supplements have unwanted effects on cell cultures. Learn how to obtain more accurate and comparable results with PromoCell’s Cancer Cell Line Medium XF.
Extended Mammosphere Culture of Human Breast Cancer CellsPromoCell 3D Tumorsphere Medium XF allows robust 3D culture even when using breast-cancer cell lines. Mammospheres are now regarded as highly valuable in vitro models for studies on tumor formation and metastasis.
Tumorsphere Culture of Cancer Stem Cells (CSC) with the PromoCell 3D Tumorsphere Medium XFFind out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Extended Neurosphere Culture of Brain Tumor Stem Cells with the PromoCell 3D Tumorsphere Medium XFUsing 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Determining Tumorsphere Formation Efficiency (TFE) with the PromoCell 3D Tumorsphere Medium XFDetermine the tumorsphere formation efficiency using an assay to quantify the ability of single cells to form spheres. Our defined and xeno-free 3D Tumorsphere Medium enables you to cultivate cancer stem cells.
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