Calcein AM

Name: Calcein AM
SKU: PK-CA707-80011
Price: 229.00 USD
Availability: InStock

Viable cell stain (cytosol); acetoxymethyl ester

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Calcein AM

PK-CA707-80011

1 mg

$229.00

Manual MSDS

Data & Figures

Figure 1. Primary human fibroblasts (NHDF) stained with Calcein AM and counterstained with DAPI.

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Reference Literature (6)
- Cell-free carrier system for localized delivery of peripheral blood cell-derived engineered factor signaling: towards development of a one-step device for autologous angiogenic therapy
  
  Hadjipanayi et al.; J Control Release. 2013 Apr 19;169(1-2):91-102
- Critical role of reactive oxygen species (ROS) for synergistic enhancement of apoptosis by vemurafenib and the potassium channel inhibitor TRAM-34 in melanoma cells
  
  Bauer et al.; Cell Death Dis. 2017 Feb 2;8(2):e2594
- CXCL1-mediated interaction of cancer cells with tumor-associated macrophages and cancer-associated fibroblasts promotes tumor progression in human bladder cancer
  
  Miyake et al.; Neoplasia. 2016 Oct;18(10):636-646
- Clinical significance of alpha- and beta-Klotho in urothelial carcinoma of the bladder
  
  Hori et al.; Oncol Rep. 2016 Oct;36(4):2117-25
- The cytoprotective effect of biglycan core protein involves toll-like receptor 4 signaling in cardiomyocytes
  
  Gaspar et al.; J Mol Cell Cardiol. 2016 Aug 9;99:138-150
- Morphological and histomorphological structures of testes and ovaries in early developmental stages of the silkworm, Bombyx mori
  Sakai et al.; J Insect Biotechnol Sericol. 2016:85:15-20
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Blog (27)

August 27, 2019Blog, Cells in action

Invisible Attraction: Using Magnets to Develop Drugs in Cell Cultures

Modern medicines are high-tech products. Developing medicines increasingly includes high throughput screening by fully automated analysis robots. One exciting high throughput approach uses magnetic 3D cell culture – which provides a more realistic model of human tissue than with 2D cell culture.
The later a candidate drops out, the more expensive it is….
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April 30, 2019Cells in action

Holotomography: 3D Non-Invasive Live-Cell Imaging of Stem Cells

Real-time tracking of stem cells during cell culture experiments can provide fundamental new insights into their biology. Yet stem cells are very sensitive to experiment-induced stress, and the use of labeling agents or intense light can damage them. With holotomography technology, long-term 3D live-cell imaging of stem cells under physiological conditions is possible – without the use of exogenous dyes and with no phototoxicity….
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January 29, 2018In the lab

Antibiotics in Cell Culture: Friend or Enemy?

Antibiotics are routinely used in cell cultures to prevent bacterial infections. But there are side effects: Studies show that they impair cell growth and differentiation. With good laboratory practice, the use of antibiotics is unnecessary.
A cell culture is a world of its own. The culture dish contains nutrients within the media that cells need to thrive and prosper….
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June 28, 2019In the lab

Scaling up from 2D monolayers to complex 3D cell cultures

Using three-dimensional cell cultures is more popular today than ever. Yet as the complexity of cell culture increases – from 2D cell culture, spheroids, 3D models up to organoids – so do the variables. This means you should plan carefully before advancing from 2D to 3D cell culture. Keeping several key factors in mind,…
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May 29, 2017In the lab

Mycoplasma Contamination – Small Organisms Cause Big Trouble

An invisible enemy that reduces the growth rate of your cell lines, mycoplasma threaten your hard work and scientific data. Every fourth cell culture could be affected. But there is hope. Our expert, Dr. Tobi Limke, explains how you can prevent mycoplasma contamination, and she outlines ways to deal with bacteria once they have infiltrated your plates and flasks….
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December 11, 2017Cells in action

Primary Cancer Cells: A Culture System Opens the Door to Individualized Therapy

Self-destruction of the body: Cancer turns normal body cells into their malignant counterpart that wants to keep on replicating by any means. Understanding the individual disease offers the potential for more effective therapies with fewer side effects. The new PromoCell Primary Cancer Culture System allows researchers to generate high-fidelity in vitro models of primary tumors….
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August 13, 2018Behind the Science

Stem Cell Therapy Scientist: Finding Inspiration Outside His Comfort Zone

Crossing the border between medical and biomechanical research fuels the research of stem cell specialist Dr. Diego Correa. We talked to the Colombian-born stem cell scientist, a former clinician with training in mechanical engineering, about his studies with mesenchymal stem cells and their potential clinical applications in cell-based therapy. Soon Correa is going to publish what he believes will be his most important contribution to this field….
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June 2, 2017Behind the Science

How Oxygen Affects Primary Cell Culture

Oxygen has two faces: While it’s a life-saving gas essential for humans and animals, excessive generation of reactive oxygen species damages cells and promotes senescence. This duality is the research focus of Professor Giovanni E. Mann of King’s College London. Prof. Mann and his colleagues are currently investigating redox signaling in vascular cells in culture….
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May 28, 2019Cells in action

Malignant Mesothelioma: Paving the Way for New Therapies

From bench to bedside and back: This is the direction of malignant mesothelioma research. Yet to understand the biology of this particular type of cancer, more basic insights are still needed. And translating that knowledge into new therapies is extremely important, as only limited therapeutic options exist so far.
Molecular needles that cross the lung epithelium: That’s how asbestos – often in form of dust – enters lung tissue (Carbone et al.,…
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December 12, 2018Cells in action

From Disease to Healing: Exploring the Roles of Pericytes

They are multipotent, interact closely with endothelial cells, and are involved in immunosurveillance: the heterogeneous group of pericytes. These cells build the wall of blood vessels, and play a crucial role in the formation and maintenance of the vasculature. Due to their involvement in various pathophysiologies such as tumor angiogenesis, scientists are beginning to exploit their potential for therapeutic approaches….
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