Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange. BrdU monoclonal antibody, is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
|Host Subclass||Mouse IgG1k|
|Immunogen||BrdU conjugated to KLH|
|Reactive Species||all species|
|Formulation||Protein A-purified antibody in PBS (pH 7.40) containing 0.02% sodium azide.|
|Reconstitution||Can be diluted in other aqueous buffers such as PBS.|
|Stability & Storage||Store at 2°C for 2 weeks. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|