BCECF
2′,7′ -bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein; fluorescent pH indicator & viable cell stain (cytosol)
BCECF
PK-CA707-51010
1 mg
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Technical Library (2)
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Does WST-8 (CCVK-I) stain viable cells?
No, it does not stain viable cells because a water-soluble tetrazolium salt (WST-8) is used for the solution. The electron mediator, 1-Methoxy PMS, receives electrons from viable cells and transfers the electrons to WST-8 in the culture medium. Since its formazan dye is also highly water soluble, CCVK-I cannot be utilized for cell staining.
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Could you send me additional information on how to use Calcein-AM?
Calcein AM (MW: 994.87) is a cell membrane-permeable, non-fluorescent dye that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein. Since esterases are only active in viable cells, the green fluorescence is directly proportional to the number of live cells in culture.
General Staining Procedure:1. Prepare 1 mM Calcein-AM solution with DMSO and dilute to prepare 1-50 µM Calcein-AM solution with PBS.2. Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture.3. Incubate the cell at 37ºC for 15-30 minutes.4. Wash cells twice with PBS or an appropriate buffer.5. Observe the cells using a fluorescence microscope with 490 nm excitation and 515 nm emission filters.Related Links and Documents
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Does WST-8 (CCVK-I) stain viable cells?
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Reference Literature (10)
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Cell-free carrier system for localized delivery of peripheral blood cell-derived engineered factor signaling: towards development of a one-step device for autologous angiogenic therapy
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Critical role of reactive oxygen species (ROS) for synergistic enhancement of apoptosis by vemurafenib and the potassium channel inhibitor TRAM-34 in melanoma cells
Bauer et al.; Cell Death Dis. 2017 Feb 2;8(2):e2594
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CXCL1-mediated interaction of cancer cells with tumor-associated macrophages and cancer-associated fibroblasts promotes tumor progression in human bladder cancer
Miyake et al.; Neoplasia. 2016 Oct;18(10):636-646
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A transient cell-shielding method for viable MSC delivery within hydrophobic scaffolds polymerized in situ
Guo et al.; Biomaterials. 2015 Jun;54:21-33
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Integrin-linked kinase (ILK) deletion disrupts oligodendrocyte development by altering cell cycle
Hussain and Macklin. J Neurosci. 2017 Jan 11;37(2):397-412
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Cellular uptake of coumarin-6 under microfluidic conditions into HCE-T cells from nanoscale formulations
Pretor et al.; Mol Pharm. 2015 Jan 5;12(1):34-45
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Clinical significance of alpha- and beta-Klotho in urothelial carcinoma of the bladder
Hori et al.; Oncol Rep. 2016 Oct;36(4):2117-25
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Thermal stress depletes energy reserves in Drosophila
Klepsatel et al.; Sci Rep. 2016; 6: 33667
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The cytoprotective effect of biglycan core protein involves toll-like receptor 4 signaling in cardiomyocytes
Gaspar et al.; J Mol Cell Cardiol. 2016 Aug 9;99:138-150
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Morphological and histomorphological structures of testes and ovaries in early developmental stages of the silkworm, Bombyx mori
Sakai et al.; J Insect Biotechnol Sericol. 2016:85:15-20
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