BCECF AM Solution (1 mg/ml in dry DMSO)

Name: BCECF AM Solution (1 mg/ml in dry DMSO)
SKU: PK-CA707-51009
Price: 119.00 EUR
Availability: InStock

Fluorescent pH indicator & viable cell stain (cytosol); acetoxymethyl ester (1 mg/ml in dry DMSO)

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PK-CA707-51009

1 mg

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Manual MSDS

Technical Library (2)
- Does WST-8 (CCVK-I) stain viable cells?
  No, it does not stain viable cells because a water-soluble tetrazolium salt (WST-8) is used for the solution. The electron mediator, 1-Methoxy PMS, receives electrons from viable cells and transfers the electrons to WST-8 in the culture medium. Since its formazan dye is also highly water soluble, CCVK-I cannot be utilized for cell staining.
  Related Links and Documents
  
  Colorimetric Cell Viability Kit I (WST-8)
- Could you send me additional information on how to use Calcein-AM?
  Calcein AM (MW: 994.87) is a cell membrane-permeable, non-fluorescent dye that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein. Since esterases are only active in viable cells, the green fluorescence is directly proportional to the number of live cells in culture.
  General Staining Procedure:1. Prepare 1 mM Calcein-AM solution with DMSO and dilute to prepare 1-50 µM Calcein-AM solution with PBS.2. Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture.3. Incubate the cell at 37ºC for 15-30 minutes.4. Wash cells twice with PBS or an appropriate buffer.5. Observe the cells using a fluorescence microscope with 490 nm excitation and 515 nm emission filters.
  Related Links and Documents
  
  Calcein AM
  
  Live/Dead Cell Staining Kit II
  
  Kurs Zellviabilitäts-, Proliferations- und Toxizitätstests (German)
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Blog (19)

January 29, 2018In the lab

Antibiotics in Cell Culture: Friend or Enemy?

Antibiotics are routinely used in cell cultures to prevent bacterial infections. But there are side effects: Studies show that they impair cell growth and differentiation. With good laboratory practice, the use of antibiotics is unnecessary.
A cell culture is a world of its own. The culture dish contains nutrients within the media that cells need to thrive and prosper….
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May 29, 2017In the lab

Mycoplasma Contamination – Small Organisms Cause Big Trouble

An invisible enemy that reduces the growth rate of your cell lines, mycoplasma threaten your hard work and scientific data. Every fourth cell culture could be affected. But there is hope. Our expert, Dr. Tobi Limke, explains how you can prevent mycoplasma contamination, and she outlines ways to deal with bacteria once they have infiltrated your plates and flasks….
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December 11, 2017Cells in action

Primary Cancer Cells: A Culture System Opens the Door to Individualized Therapy

Self-destruction of the body: Cancer turns normal body cells into their malignant counterpart that wants to keep on replicating by any means. Understanding the individual disease offers the potential for more effective therapies with fewer side effects. The new PromoCell Primary Cancer Culture System allows researchers to generate high-fidelity in vitro models of primary tumors….
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August 13, 2018Behind the Science

Stem Cell Therapy Scientist: Finding Inspiration Outside His Comfort Zone

Crossing the border between medical and biomechanical research fuels the research of stem cell specialist Dr. Diego Correa. We talked to the Colombian-born stem cell scientist, a former clinician with training in mechanical engineering, about his studies with mesenchymal stem cells and their potential clinical applications in cell-based therapy. Soon Correa is going to publish what he believes will be his most important contribution to this field….
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June 2, 2017Behind the Science

How Oxygen Affects Primary Cell Culture

Oxygen has two faces: While it’s a life-saving gas essential for humans and animals, excessive generation of reactive oxygen species damages cells and promotes senescence. This duality is the research focus of Professor Giovanni E. Mann of King’s College London. Prof. Mann and his colleagues are currently investigating redox signaling in vascular cells in culture….
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April 17, 2018In the lab

A Home for Cancer Stem Cells:
3D Tumorspheres

Many scientists working in oncology focus on cancer stem cells (CSCs). Targeting these cells specifically could improve cancer therapy significantly. Because CSCs rely heavily on their environment, three-dimensional cultivation offers the unique opportunity to better understand this interaction. We talked to two scientists working in this field of cancer research.
In the human body,…
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June 8, 2017Cells in action

Why You Should Know Your Human Mesenchymal Stem Cells Inside Out

Doctors, and patients with chronic diseases, are putting hope into new findings in regenerative medicine that are based on mesenchymal stem cell (MSC) research. As promising as some published results sound, they might be not reproducible – simply due to insufficient authentication and poor handling of cell cultures. Rigorous validation of cell identity is crucial for working with human mesenchymal stem cells – and,…
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August 31, 2017In the lab

Trouble With Your Primary Cells? Treat Them Nicely!

What seems basic to cell culturing is pure stress for primary cells: thawing, subcultivation and freezing. Even seasoned scientists tend to overlook this aspect, and manipulate their cultures less carefully than they should. Unhealthy, or even dead cells are the sad consequence. Here, you can benefit from an expert’s tips in handling primary cells,…
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January 10, 2018Cells in action

Some Human Mesenchymal Stem Cells are More Equal than Others – Origins and Differences

It is a scientific success story that started approximately 40 years ago – and was initially met with skepticism. Although the pioneers of mesenchymal stem cell research were initially criticized, today thousands of publications and hundreds of clinical trials focus on multipotent cells. New research is suggesting that in the end,…
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April 5, 2018Cells in action

Mimicking the Body’s Largest Organ In Vitro with 3D Skin Models

Researchers are increasingly using 3D cell cultures to study the skin’s features. Three-dimensional skin models offer the potential to study various diseases and test compounds in vitro. Dr. Florian Groeber-Becker from the Fraunhofer Institute for Silicate Research ISC in Wurzburg has developed a highly sophisticated skin model.
It is the first thing people see when they look at us….
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