Autophagy/Cytotoxicity Dual Staining Kit
Allows to study the regulation of autophagy and cytotoxicity in cultured cells by fluorescence microscopy and flow cytometry.
Autophagy or autophagocytosis (“self-eating”) refers to a process of degradation of cytoplasmic components within lysosomes and consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. This process is mediated by autophagosomes which engulf a portion of cytoplasm. Thus, autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases.
The Autophagy/Cytotoxicity Dual Staining Kit enables detection and monitoring of cellular autophagy processes and autophagic cell death/cytotoxicity using a membrane-permeable, selective autophagy stain and a fluorescent cell death marker. The kit can also be used for screening of compounds affecting autophagy and results are obtained using fluorescence microscopy or flow cytometry.
An autophagy-inducing positive control reagent which increases autophagy staining serves as an experimental control.