Aspartate Aminotransferase (AST/SGOT) Activity Assay Kit
Rapid, simple, and sensitive colorimetric high-throughput assay for measuring AST/ SGOT activity in various biological samples.
The PromoKine Aspartate Aminotransferase Activity Assay Kit provides a rapid, simple, sensitive and reliable test that is suitable for high throughput applications. In a reversible transamination reaction, AST catalyzes the Transfer of an amino Group from aspartate to ⍺-ketoglutarate, forming oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to a colored product (λmax = 450 nm). Absorbance directly correlates to AST activity in the sample.