Activin A, human, recombinant (InC)

Recombinant Human/Mouse/Rat Activin A (insect cell-derived)

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Activin A, human, recombinant (InC)
C-60057
  10 µg
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Activin A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin. Human Activin A is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues.
Please note: Recombinant human Activin A is identical to recombinant mouse and rat Activin A as numerous literary sources demonstrating the complete identity of the mature Activin A sequence among these three species.

Molecular Mass 26 kDa
Source High-5 insect cells
Specific Activity ≥ 5 x 10⁵ IU/mg
Biological Activity The ED₅₀ as determined by its ability to inhibit the proliferation of murine MPC-11 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.
Formulation Lyophilized from a sterile-filtered solution with no additives.
Reconstitution Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Purity Greater than 95% (determined by SDS-PAGE and HPLC analysis).
Endotoxin Level < 0.1 ng per µg of rHu Activin A (1EU/µg).
Stability & Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Activin A should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.