Activin A, human, recombinant (HEK)

Recombinant Human Activin A (HEK cell-derived)

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Activin A, human, recombinant (HEK)
C-60061
  10 µg
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Activin A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin.
Human recombinant Activin A produced in HEK cells is a non-glycosylated disulfide-linked homodimer, having a total molecular weight of 25 kDa. It has been purified by proprietary chromatographic techniques.
Please note: Recombinant human Activin A is identical to recombinant mouse and rat Activin A as numerous literary sources demonstrating the complete identity of the mature Activin A sequence among these three species.

Molecular Mass 25 kDa
Source HEK cells
Specific Activity see below
Biological Activity The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line) and is typically 0.5-5 ng/ml.
Formulation Lyophilized from a sterile-filtered protein solution in 1XPBS with no additives.
Reconstitution Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Purity Greater than 95% (determined by SDS-PAGE analysis).
Endotoxin Level < 0.1 ng per µg of rHu Activin A (1EU/µg).
Stability & Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Activin A should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles.