Activin A, human, recombinant (E. coli)
Recombinant Human/Mouse/Rat Activin A (insect cell-derived)
Activin A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin.
Recombinant human Activin A produced in E. coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2 kDa.
Recombinant Activin A has been purified by proprietary chromatographic techniques.
Please note: Recombinant human Activin A is identical to recombinant mouse and rat Activin A as numerous literary sources demonstrating the complete identity of the mature Activin A sequence among these three species.
|Molecular Mass||26.2 kDa|
|Specific Activity||2.6 x x 10⁵ IU/mg|
|Biological Activity||Biological activity is assessed by the ability to induce cytoxicity of MPC-11 cells and was found to be 3.8 ng/ml corresponding to a specific activity of 2.6 x x 10⁵ units/mg.|
|Formulation||Human Activin A was lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution containing 0.1% TFA.|
|Reconstitution||Please Note: Always centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.|
|Purity||Greater than 95% (determined by SDS-PAGE analysis).|
|Endotoxin Level||< 0.1 ng per µg of rHu Activin A (1EU/µg).|
|Stability & Storage||The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BNP should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.|