Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Murine adiponectin shares about 83% amino acid identity with that of human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
The mouse Acrp30 is produced in E. coli as a recombinant protein with an N-terminal His-Tag. The His-tagged fusion protein has a MW of 27 kDa containing amino acid residues 18-247 of the mouse Acrp30.
|Molecular Mass||27 kDa|
|Biological Activity||Measured by its ability to inhibit proliferation of mouse myeloid cell line M1 (Yokota, T. et al., Blood 96:1,723, 2,000). The ED₅₀ for the effect is typically 1 μg/ml.|
|Formulation||Mouse Acrp30 is a sterile-filtered, liquid formulation containing 20 mM Tris (pH 8), 1 mM DTT and 10% glycerol.|
|Reconstitution||Please Note: Always centrifuge product briefly before opening vial. The protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.|
|Purity||Greater than 90% (determined by SDS-PAGE).|
|Endotoxin Level||< 0.10 ng per µg rMu Acrp30 (1EU/µg).|
|Stability & Storage||Store at 4°C if entire vial will be used within 1 week. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.|