Acrp30, human, recombinant (Sf9)

Recombinant Human Adiponectin (Adipocyte Complement Related Protein of 30 kDa; Hi-5 insect cell-derived)

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Acrp30, human, recombinant (Sf9)
C-66629
  25 µg
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Adiponectin is an adipose-derived secreted protein containing 226 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant adiponectin is a multimeric glycoprotein containing amino acids Glu-19 to Asn-244 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions.

Molecular Mass 35.0 kDa
Source Insect cells
Specific Activity see below
Biological Activity Determined by a cytotoxic assay using M1 cells. The ED₅₀ for this effect is 3.0-6.0 μg/ml.
Formulation Lyophilized from a sterile-filtered solution with no additives.
Reconstitution Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1 – 1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Purity Greater than 97% (determined by SDS-PAGE and HPLC analysis)
Endotoxin Level < 0.1 ng per µg of rHu Acrp30 (1EU/µg).
Stability & Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.