Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (adipokine). The protein belongs to the soluble defence collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
Human recombinant Acrp30 produced in HEK cells is fused to a FLAG tag and purified using proprietary chromatographic techniques.
|Molecular Mass||30 kDa|
|Specific Activity||see below|
|Biological Activity||In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED₅₀ was ~ µg/ml.|
|Formulation||White, lyophilized (freeze-dried) powder. Lyophilized from a sterile-filtered (0.40 µm) protein solution (0.50 mg/ml) in 20 mM Tris buffer (pH 7.50) 20 mM NaCl.|
|Reconstitution||Please Note: Always centrifuge product briefly before opening vial. For reconstitution, prepare a working stock solution of approximately 0.50 mg/ml with deionized water and let the lyophilized pellet dissolve completely. This solution should be steril-filtered again using an appropriate sterile filter and can then be stored at -20°C for future use.|
|Purity||Greater than 98% (determined by SDS-PAGE and HPLC analysis).|
|Stability & Storage||The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Acrp30-HEK should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.|