5X Annexin V Binding Buffer
Concentrated Annexin V Binding Buffer to be used for Annexin V staining.
The human anticoagulant Annexin V is a 35-36 kDa, Ca²+-dependent phospholipid-binding protein with high affinity for phophatidylserine (PS). During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface where it can be detected by labeled Annexin V which is a common probe for detecting apoptosis. 5X Annexin V Binding Buffer is a concentrated stock solution used to prepare 1X Annexin V Binding Buffer which is optimized for staining cells with Annexin V conjugates.