The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5’-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation, at least in part by inhibiting the activity of TOR towards 4E-BP1.
|Host Subclass||Rabbit IgG|
|Immunogen||Rabbit polyclonal 4E-BP1 antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1 (Genbank accession No. NP_940995). Anti-4E-BP1 is human and mouse reactive.|
|Reactive Species||Human, Mouse|
|Formulation||Antibody is supplied in PBS containing 0.02% sodium azide.|
|Reconstitution||During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.|
|Stability & Storage||Antibody can be stored at 4°C for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|References||Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2,000; 279:E715-29.|