3D Tumorsphere Medium XF
Xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.
The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
- Establish 3D tumorsphere cultures directly from 2D cancer cell lines
- Suitable for long-term routine culture of tumorspheres
- Compatible with most commonly used cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:
|Tissue||Tested Cell Lines||Cell Lines Origin|
|Brain||U-87 MG||grade IV glioblastoma / astrocytoma of the human brain|
|Breast||MCF-7||pleural effusion of metastatic human breast adenocarcinoma|
|Breast||MDA-MB-231||pleural effusion of metastatic human breast adenocarcinoma (triple-negative)|
|Colon||HT-29||human colon adenocarcinoma|
|Connective tissue||HT1080||human fibrosarcoma|
|Liver||HepG2||hepatocellular carcinoma of the human liver|
|Lung||A-549||human lung carcinoma|
|Pancreas||Panc-1||epithelioid carcinoma of the human pancreatic duct|
|Prostate||LNCaP||lymph node metastasis of human prostate adenocarcinoma|
|Skin||A-431||epidermoid carcinoma of the human skin|
Technical Library (2)
Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
During our quality control we do not determine the presence or maintenance of respective stem cell markers in the tumorspheres. We use a functional approach instead, by culturing the tumorspheres over serial passages in C-28070. Tumorsphere formation requires the biological features of Anoikis resistance and self-renewal and therefore indicates the presence of cancer stem cells (CSC)/cancer initiating cells (CIC) in the spheres. 3D tumorsphere culture thus allows scientists to study the biology of CSCs without any background knowledge on CSC markers.
Related Links and Documents
Can I use the 3D Tumorsphere Medium XF for mouse cell lines?
Yes, we have received a customer feedback that our 3D Tumorsphere Medium XF (C-28070) has been successfully used for tumorsphere formation of mouse cell lines.
Related Links and Documents
- Does the PromoCell 3D Tumorsphere Medium XF (C-28070) maintain the stem cell character of cancer stem cells?
Application Notes (5)
Determining Tumorsphere Formation Efficiency (TFE) with the PromoCell 3D Tumorsphere Medium XFDetermine the tumorsphere formation efficiency using an assay to quantify the ability of single cells to form spheres. Our defined and xeno-free 3D Tumorsphere Medium enables you to cultivate cancer stem cells.
Tumorsphere Culture of Cancer Stem Cells (CSC) with the PromoCell 3D Tumorsphere Medium XFFind out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Extended Neurosphere Culture of Brain Tumor Stem Cells with the PromoCell 3D Tumorsphere Medium XFUsing 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Extended Mammosphere Culture of Human Breast Cancer CellsPromoCell 3D Tumorsphere Medium XF allows robust 3D culture even when using breast-cancer cell lines. Mammospheres are now regarded as highly valuable in vitro models for studies on tumor formation and metastasis.
Reference Literature (2)
Poly-lactic-co-glycolic acid nanoformulation of small molecule antagonist GANT61 for cancer annihilation by modulating hedgehog pathway
Borah et al.; NanoWorld J. 2017;3(1):1-10
Down-regulation of BORIS/CTCFL efficiently regulates cancer stemness and metastasis in MYCN amplified neuroblastoma cell line by modulating Wnt/beta-catenin signaling pathway
Garikapati et al.; Biochem Biophys Res Commun. 2017 Feb 26;484(1):93-99
- Poly-lactic-co-glycolic acid nanoformulation of small molecule antagonist GANT61 for cancer annihilation by modulating hedgehog pathway
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