3D Tumorsphere Medium XF
Xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.
The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
- Establish 3D tumorsphere cultures directly from 2D cancer cell lines
- Suitable for long-term routine culture of tumorspheres
- Compatible with most commonly used cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:
|Tissue||Tested Cell Lines||Cell Lines Origin|
|Brain||U-87 MG||grade IV glioblastoma / astrocytoma of the human brain|
|Breast||MCF-7||pleural effusion of metastatic human breast adenocarcinoma|
|Breast||MDA-MB-231||pleural effusion of metastatic human breast adenocarcinoma (triple-negative)|
|Colon||HT-29||human colon adenocarcinoma|
|Connective tissue||HT1080||human fibrosarcoma|
|Liver||HepG2||hepatocellular carcinoma of the human liver|
|Lung||A-549||human lung carcinoma|
|Pancreas||Panc-1||epithelioid carcinoma of the human pancreatic duct|
|Prostate||LNCaP||lymph node metastasis of human prostate adenocarcinoma|
|Skin||A-431||epidermoid carcinoma of the human skin|