3D Tumorsphere Medium XF

Xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.

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The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.

Components:

The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Key features:

  • Establish 3D tumorsphere cultures directly from 2D cancer cell lines
  • Suitable for long-term routine culture of tumorspheres
  • Compatible with most commonly used cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:

Tissue Tested Cell Lines Cell Lines Origin
Brain U-87 MG grade IV glioblastoma / astrocytoma of the human brain
Breast MCF-7 pleural effusion of metastatic human breast adenocarcinoma
Breast MDA-MB-231 pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
Colon HT-29 human colon adenocarcinoma
Connective tissue HT1080 human fibrosarcoma
Liver HepG2 hepatocellular carcinoma of the human liver
Lung A-549 human lung carcinoma
Pancreas Panc-1 epithelioid carcinoma of the human pancreatic duct
Prostate LNCaP lymph node metastasis of human prostate adenocarcinoma
Skin A-431 epidermoid carcinoma of the human skin
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
Figure 4. List of some cell types successfully tested for serial passage with the PromoCell 3D Tumorsphere Medium XF.

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