Pulmonary Cell Culture
The human lung consists of several different cell types with highly specialized functions. Our comprehensive portfolio of primary human pulmonary cell types allows to model the characteristics of each cell type to investigate their role in developmental biological processes, pharmacological studies, and disease modeling.
Airway epithelial cells
PromoCell offers epithelial cells isolated from the nasal mucosa, trachea, bronchi, and distal respiratory tract of individual donors. Flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot.
Pulmonary fibroblasts
Our primary Human Pulmonary Fibroblasts are isolated from human lung tissue. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot.
Respiratory tract smooth muscle cells
Airway smooth muscle cells are available from bronchial and tracheal tissue of individual donors. Each lot is routinely tested for expression of smooth muscle alpha-actin.
Pulmonary microvascular endothelial cells
PromoCell’s primary Human Pulmonary Microvascular Endothelial Cells are isolated from lung tissue of individual donors. They comprise a mixtures of blood and lymphatic endothelial cells. Each produved lot is analyzed for CD31 and von Willebrand factor expression and Dil-Ac-LDL uptake.
Donor information
All our cell types are sourced from healthy, consented donors. Specific donor information like age, sex and tissue location is available for each produced lot. Cells from diseased donors (e.g. asthma, COPD) are available upon request.
Quality control
In addition to cell type-specific marker analysis, each produced lot is tested for growth performance through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).