Microbial Detection, Elimination & Prevention
Cell culture contamination is a commonly occurring problem that affects many of the cell’s biochemical and immunological properties, and it leads to unsatisfactory and unreliable results. Therefore, regularly testing cell cultures for contamination with mycoplasmas or bacteria is essential. PromoCell provides highly sensitive and user-friendly test kits for this purpose as well as products for the effective elimination and prevention of mycoplasma and eubacteria contamination.
Detection
The PromoKine PCR Mycoplasma Test Kits are convenient PCR based kits for fast, ultra-sensitive and reliable detection of contaminating mycoplasmas in various biological materials including cell cultures and virus stocks. They are very user friendly and contain all components required for performing successful PCR tests for mycoplasma contamination following an easy protocol for sample preparation and PCR. The kits are available for conventional PCR and Real Time qPCR and detect very specifically more than 20 mycoplasma and acholeplasma species with highest sensitivity.
In Addition, a PCR Bacteria Test Kit for fast, accurate and reliable detection of 45 eubacteria (many of them usually found as contaminants in cell culture) is available as well.
Elimination
PromoCell provides reagents and kits for elimination of mycoplasmas contaminating your cell cultures or virus stocks. The PromoKine Mycoplasma-EX Kit consists of a non-antibiotic, mycoplasma killing reagent and an antibiotic mycoplasma growth inhibiting solution. This combination has been shown to be highly active in the thorough elimination of mycoplasma species that are responsible for more than 90% of the contaminations found in cell cultures. When used according to our instructions, an extremely efficient mycoplasma elimination will be achieved within a few days without any no cytotoxic effects or cell alteration. In addition, classical antibiotic solutions (BIOMYC) highly effective against mycoplasmas in cell culture are also available.
Prevention
To be always on the safe side and prevent reliably any microbial contamination of your cell culture lab, you may use PromoCell’s range of highly efficient, cost-effective and virtually non-hazardous disinfection reagents suited to clean and disinfect lab surfaces fast (sprays and wipes) and to maintain/stabilize the water in ordinary water baths or CO2 incubator water trays.