Mesenchymal Stem Cell Culture
Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages.
Primary human mesenchymal stem cells
PromoCell’s human Mesenchymal Stem Cells (hMSC) are available as cryopreserved or proliferating cells isolated from bone marrow, adipose tissue, and umbilical cord matrix of individual donors. All cells verified to have MSC expression profiles in accordance with ISCT* guidelines.
Mesenchymal stem cell growth media
PromoCell MSC Growth Media robustly support the expansion of multipotent human MSC while retaining maximum multipotency throughout numerous passages. Our growth media is available as low-serum (Mesenchymal Stem Cell Growth Medium 2) and serum-free/xeno-free (Mesenchymal Stem Cell Growth Medium DXF) for applications requiring defined cell culture conditions.
Differentiation media
PromoCell’s MSC Differentiation Media allow for directed and efficient differentiation of human MSC into adipocytes, osteoblasts, chondrocytes and neurons. Detailed step-by-step protocols are available in our Application Notes on our website.
Quality control and characterization
PromoCell’s human MSC are isolated in accordance to the highest ethical standards and extensively quality tested including marker characterization, growth performance, and in vitro differentiation. Each produced MSC lot is stringently tested for:
- Cellular markers: positive for CD73, CD90, CD105, negative for CD14, CD19, CD34, CD45, HLA-DR expression (in accordance with ISCT criteria*).
- Differentiation potential into chondrogenic, apidogenic and osteogenic lineages.
- Growth performance and morphology during initial 10 population doublings
*ISCT (International Society for Cellular Therapy) Cytotherapy (2006) Vol. 8, No. 4, 315-317