Endothelial Cell Culture
The in vitro culture of primary human endothelial cells is a widely established model system used to investigate several cardiovascular diseases and cancer research applications. Our comprehensive endothelial cell culture portfolio consists of 12 different types of large vessel and microvascular human endothelial cells with optimized growth media for each cell type.
Tissue sources and donor information
PromoCell large vessel endothelial cells are available from the umbilical vein and artery, the aorta, the coronary artery, the pulmonary artery, and the saphenous vein. Our microvascular endothelial cells are available from dermal, lung, cardiac and uterine tissues. All our cell types are sourced from healthy, consented donors. Specific donor information like age, sex and tissue location is available for each produced lot. Endothelial cells from diseased donors (e.g. diabetes, myopathies) are available upon request.
Cell culture media – optimized for each cell type
Just like the cellular microenvironment is different for large and microvascular vessels, such are their media requirements. At PromoCell we have developed optimized growth media for both large vessel and microvascular endothelial cell types. In addition, we offer media formulations with and without Endothelial Cell Growth Supplement (ECGS), a bovine hypothalamic extract traditionally used for cultivating endothelial cells without added VEGF. Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on the cell metabolism, it may also interfere with certain experimental setups.
Quality control
Each produced cell lot is tested for cell morphology, adherence rate, cell viability, flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Explore Portfolio
Human Endothelial Cells
- Human Umbilical Vein Endothelial Cells (HUVEC)
- Human Umbilical Artery Endothelial Cells (HUAEC)
- Human Aortic Endothelial Cells (HAoEC)
- Human Coronary Artery Endothelial Cells (HCAEC)
- Human Pulmonary Artery Endothelial Cells (HPAEC)
- Human Saphenous Vein Endothelial Cells (HSaVEC)
- Human Dermal Microvascular Endothelial Cells (HDMEC)
- Human Dermal Blood Endothelial Cells (HDBEC)
- Human Dermal Lymphatic Endothelial Cells (HDLEC)
- Human Cardiac Microvascular Endothelial Cells (HCMEC)
- Human Pulmonary Microvascular Endothelial Cells (HPMEC)
- Human Uterine Microvascular Endothelial Cells (HUtMEC)