Have a question about one of our products? Check out our technical library for recently asked questions from other scientists around the world.
Technical Library
Recent Entries in Technical Library
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Is the PromoCell Keratinocyte Growth Medium 2 suitable for growth of primary mouse keratinocytes?
Yes, primary mouse keratinocytes grow well in this media when you reduce the CaCl2 concentration to 0.025 mM – 0.05 mM CaCl2 (optimal Ca concentration for primary human keratinocytes is 0.06 mM).
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When do I need PromoCell’s Monocyte Attachment Medium (C-28051)?
The Monocyte Attachment Medium allows for the efficient adherence selection (within 1 hr) of monocytes from freshly isolated mononuclear cells while maintaining optimal cell health. The Time-consuming and costly immunomagnetic purification of monocytes prior to DC generation is not necessary and can be skipped when using this medium.
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How long does it take to detach primary cells with accutase (C-41310)?
Most primary cells detach within 5-10 min at 37°C. Inactivation isn’t required but we recommend to centrifuge the cell suspension to remove accutase and EDTA before replating the cells.
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Is it possible to transfect PromoCell Normal Human Cells?
Yes, it is possible to transfect our normal human cells. In general, primary and normal cells are much harder to transfect than immortalized cell lines. Apart from the cell type, successful transfection also depends on the culture’s age and density at transfection, the vector used, the purity of the nucleic acids, the composition of the transfection medium, and the experimental conditions.
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What’s the stability of HPC Expansion Medium XF (C-28021) after addition of Cytokine Mix E (C-39890)?
The medium supplemented with Cytokine Mix E is stable for 2 weeks if stored protected from light at 2-8°C.
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Is it necessary to heat inactivate FCS for use with Normal Human Cells?
The practice of heat inactivation was originally developed when only serum from adult animals was available. Adult serum contains high serum complement which may destroy cells under certain conditions. Heating serum (30 min, 56°C) is intended to inactivate the complement. Today, serum is often heat-inactivated without any evidence of beneficial effect. When using FCS (fetal calf serum), heat inactivation is not necessary for most cell lines or cell types. PromoCell does not use heat-inactivated FCS for the preparation of their growth media.
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To what extent will the CD34+ Progenitor Cells proliferate in Hematopoietic Progenitor Cell Expansion Medium XF (C-28021)?
For optimal cell growth, the human CD34+ Progenitors (C-12921) are cultured in PromoCell Hematopoietic Progenitor Cell Expansion Medium XF (C-28021) supplemented with Cytokine Mix E (C-39890) or with any other cytokine cocktail suitable to achieve optimal cell expansion. Cytokine Mix E contains recombinant human TPO, SCF, flt3-ligand and IL-3. The strong expansion of CD34+ cells will persist for approx. 2 weeks. The expansion factor is usually between 75 and 200-fold.
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What are the criteria for selection or exclusion of peripheral blood donors? Is the peripheral blood that PromoCell use to isolate blood cells (MNC-PB, monocytes) derived from completely healthy donors?
The following criteria are applied for blood donations:
a) Donors with light infection like a cold or cough are excluded for one week
b) Donors with infections with a temperature above 37.9°C and/ or antibiotics therapy are excluded for 4 weeks
c) Blood pressure: exclusion only if systolic blood pressure is < 100 mm Hg or > 180 mm Hg or if diastolic blood pressure is > 100 mm Hg. No exclusion if blood pressure is drug treated and in acceptable range.
d) High cholesterol: no exclusion even though medication is used
e) Diabetes: exclusion if diabetes type I or use of insulin
f) Steroid use: exclusion for 4 weeks after application
g) Cancer: exclusion
h) Other chronic diseases: exclusion depends on the type of disease (for example chronic heart disease, autoimmune disease)Related Links and Documents
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What medium should I use to grow or differentiate PromoCell Human Stem and Blood Cells?
The recommended media for a particular cell type are specified on the respective product page (“Recommended Products”) and can be found in the Manual belonging to the cells.
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From what tissue do PromoCell isolate their HPMEC (C-12281)?
Our HPMEC are isolated from peripheral lung tissue of adult donors.
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