Have a question about one of our products? Check out our technical library for recently asked questions from other scientists around the world.
Technical Library
Recent Entries in Technical Library
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What are the advantages of monocyte enrichment by adherence selection using your Monocyte Attachment Media (C-28051)?
This method produces large numbers of adherent monocytes in only 1.5 h. If the washing steps are properly performed, 80-90% purity can be expected. The attached cells are “untouched”, since no binding of magnetic microbeads has occured. This also excludes phagocytosis of the microbeads by the monocytes, an event which is unfavorable with regard to cellular health. In addition, the adherence method is time-saving and cost-effective.
Related Links and Documents
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Is it normal that the cell pellets from PromoCell (C-14**) do not freeze at -20°C?
Samples in RNAlater will NOT freeze at -20°C, they are liquid at 4°C and -20°C. The samples can be stored at 4°C for up to 1 month and are stable at room temperature for up to one week. At -20°C, they can be stored indefinitely.
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What’s the difference between NHEK.f and NHEK.f pooled?
NHEK.f are isolated from tissue samples (foreskin) of single donors, aged between 1-10 years. NHEK.f pooled are prepared from the foreskins of 3 individual donors. The cells of each donor are expanded in separate TC vessels and the cells are pooled after secondary culture, before cryopreservation.
Both NHEK from single donor and NHEK pooled are in P2 after thawing.Related Links and Documents
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Do the PromoCell keratinocytes need feeder cells?
If you are growing our NHEK in PromoCell Keratinocyte Growth Medium 2 (C-20011) or Growth Medium 3 (C-20021), you don’t need any feeder cells. The cells will grow as a monolayer in conventional tissue culture flasks.
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Why does PromoCell only guarantee 15 PDs when with my own self isolated dermal fibroblasts I regularly achieve 30 passages?
The PromoCell quality control procedure includes the cultivation of fibroblasts for 15 population doublings. This is to ensure that the cells can be grown for a minimum of 15 PDs (6-8 passages depending on the split ratio used) but it does not mean that the cells immediately senesce after that point. We don’t determine the maximum number of doublings or passages but most NHDF lots will certainly achieve > 20 passages.
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What is the reason for turbid serum?
Serum turbidity is usually caused by cryoprecipitation of lipid components during freezing and thawing. The more times the serum is subjected to freeze/thaw cycles, the more turbidity is noticed. It can be minimized by freezing the serum in aliquots at -20°C and thawing these aliquots individually at the time of use.
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Can I aliquot the SupplementMix?
Yes, you can aliquot the SupplementMix upon delivery and freeze down 2 or 4 individual aliquots at -20°C. This way, you can prepare smaller volumes (2 x 250 ml or 4 x 125 ml) of complete culture medium and thus extend the time you can use the medium.
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Why does PromoCell use CD146 as a marker for pericytes?
CD146 (MUC18) is a surface marker, expressed on pericytes, MSCs, and endothelial cells from large vessels (but not microvascular endothelial cells). In combination with CD34 (a marker for endothelial and hematopoietic cells), pericytes can be characterized by FACS analysis as CD146+/CD34–. The absence of CD34 expression makes sure that the cells are not of hematopoietic/endothelial origin. Further markers that have been described for pericytes are NG2, CD90, alpha-SMA, and PDGFR-beta.
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Are the HDMEC pre-screened (C-12215) derived from juvenile foreskin or from adult skin?
All HDMEC pre-screened lots that we currently have in stock are isolated from foreskin.
If you need pre-screened HDMEC from adult donors, please contact the PromoCell Technical Customer Support.Related Links and Documents
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Is it possible to perform experimental starvation with PromoCell Normal Human Cells and the PromoCell media?
Yes, but the experimental starvation conditions have to be determined individually for each cell type. Usually, the cells are maintained in basal medium with reduced growth factor concentrations or lower FBS content. The cells need to be in a good condition and the starvation should be kept as short as possible as prolonged serum and/or growth factor deprivation induces apoptosis.
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