Products

New 2017/2018 Catalog

Your products for cell culture and cell biology in our web catalogue

At PromoCell we are constantly striving to support you and your research project to the best of our ability! To simplify your search for products and information, we have combined all of our products into just one catalog.

Included product categories:

  • Human Primary Cells & Media
  • Stem and Blood Cells & Media
  • Cell Biology Products
  • Cell Analysis
  • Cell Transfection
  • Antibodies & ELISAs

 

To view our catalog please click here!

Tell us what you think.


Updated Human Mesenchymal Stem Cell Media

PromoCell offers a complete Mesenchymal Stem Cell Media System including growth media, differentiation media and human mesenchymal stem cells (MSC).

The PromoCell MSC Growth Media are designed to robustly support the standardized expansion of multipotent human Mesenchymal Stem Cells. Rigorous quality control tests ensure reliable and consistent product quality.


Take advantage of PromoCell's state of the art MSC products for better results


Key Features

·         Guaranteed Growth Performance
All of our MSC are guaranteed to grow with a doubling time of <30h for a minimum of 10 population doublings.

·         Differentiation Tested
Each MSC lot is tested for differentiation into osteoblasts, adipocytes and chondrocytes in accordance with ISCT* guidelines.

·         ISCT* Marker Panel
Each MSC lot is tested for CD73+/CD90+/CD105+ and CD14-/CD19-/CD34-/CD45-/HLA-DR-.

·         Higher Result Comparability
Our newly designed MSC Growth Medium 2 with low-serum content (2% FCS) ensures low batch-to-batch variation.

·         Chemically Defined & Xeno-Free
DXF Medium available for chemically defined and xeno-free MSC culture.


Now available: Cancer Stem Cell Medium

Setting a New Standard with Next Generation Tumorsphere Medium!

Key Advantages:

  • Supports most commonly used cancer cell lines
  • Allows extended serial tumorsphere passages
  • High tumorsphere formation efficiency
  • Serum-free and chemically defined
  • Ready to use

 

 Click here for more information

 

 

 


PromoCells in Space – Part III: M2 Macrophages in Zero Gravity

 

Have you ever been weightless or experienced zero gravity? Our macrophages have! There are several possibilities to doing research in weightlessness – in fact, during our last two episodes of PromoCells in Space we have already introduced you to one of them: sounding rockets. However, suborbital and orbitals flights are instruments to elucidate mid- and long-term effects of microgravity, whereas ultra-short, initial and primary effects and mechanisms are amenable by the short-term microgravity provided by parabolic flight maneuvers. In consequence, man and experiment can board the Airbus A-300 ZERO-G in Bordeaux, France. On this flight you can experience 20 seconds of zero-g in free fall not just once, but 31 times in a row at an altitude of 5000 meters.


This is exactly what Prof. Ullrich from the University of Zurich and his team have been doing on a regular basis to help expand our knowledge surrounding astronauts’ ill-health when in space. Long-term manned space flights are often hindered when the astronaut begins to suffer several bodily ailments. For example astronauts are prone to infections and are also known to suffer from re-activation of dormant endoviruses. Gravity has been a constant force throughout evolutionary history on Earth. And therefore, it is one of the fundamental biological questions, if and how life on Earth requires gravity for complex cell functions. On Prof. Ullrichs team, the researchers use the parabolic flight to take a closer look at immune cells that have already been shown to be compromised by the suspension of gravity (Ullrich O and Thiel CS 2012; Stress Challenges and Immunity in Space, pp 187-202).


The team is working to find out how human immune cells sense and react to weightlessness. A chosen cell type to focus on are M2 macrophages. These cells are generated from peripheral blood mononuclear cells using PromoCell’s new Macrophage Generation Media DXF.

Being weightless is a hard job however! It means getting up at 5.30 in the morning, preparing yourself and the cells for the ride of your life, taking a dose of dazing scopolamine against parabolic flight sickness, boarding the A-300 ZERO-G and trying not to waist too much energy by getting nervous before you experience probably the most unique feeling ever: weightlessness. Parabolic flights taking off from Bordeaux are performed at an altitude of 5000 meters and take place in well-defined areas mainly over the atlantic ocean. Researchers from all over the world can apply to get the possibility of collecting data in microgravity. Each time the A-300 ZERO-G lifts off, it carries several projects from different research fields on board. It takes two pilots to operate the Airbus, one controlling the pitch of the airplane and the other being in charge of the lateral rotation called roll. Before the A-300 can enter the phase of weightlessness, it has to pull up at an angle that finally reaches 47°. During this pull up the passengers are exposed to up to 1.8 g. That’s quite a lot! At that time you can personally experience what it is like when centrifuging your cells at 200-350 g... And then, just before the peak of the parabola, the thrust is reduced and all goes quiet. After a couple of split seconds in which you ask yourself why you actually got on to this vehicle, you start free floating and know the answer: because this is the most thrilling ride of your life! The A-300 then crosses the peak and starts descending. Actually it falls without interference by the engines or brake flaps. One phase of weightlessness lasts around 20 seconds and then you are sedimented down to the floor of the aircraft by subtle onset of the pull out maneuver at 1.8 g again. At that point you are happy that you are not a cell being pelleted in a conical tube. After the pull out you get 1 minute to sort out your guts and then it starts all over again. Still coping with the situation?

Good, then you can do what you were actually recruited for, get a fresh pouch of cells out of the incubator, fix it to the tubing of the reagents supply in the experiment rack, store the old pouches in the cooling unit, secure everything including yourself and await the next butterflies swarm in your stomach. If you still don’t feel busy enough, you can expand your scope by exchanging broken needles of the tubing, hoses, clamps, insulations or whatever you are lucky to detect. Of course, this doesn’t mean that the interval between parabolas will be extended just for you. After five parabolas you start realizing that the manpower of other teams seems to be decreasing and after ten parabolas you discover the sick bay with very poorly looking people in the tail of the plane. Very briefly you think about joining them. However, after 12 parabolas you somehow get used to it, although you notice how exhausting this sequential sedimentation is starting to be, and after 15 parabolas you actually begin liking the feeling of being superman. And finally, by the 30th parabola you are sad that there’s only one more to go. After landing, there is still the lab job waiting for you. Unfortunately, macrophages differentiated with PromoCell Macrophage Generation Media DXF don’t prepare their RNA by themselves, that’s one thing that can still be improved until next time!

 

Copyright by Novespace

19th DLR Parabolic Flight Campaign: Signal Transduction in Cells of the Immune System in Microgravity (SITI-3).

The experimental racks are mounted into the Airbus A-300 ZERO-G.

Control of the assembly by the respective team members.

These are the racks in which the M2 macrophages will be flying. In the back you can see the incubator, on the right hand side is the experimental rack and on the left the cooling unit.

In the evening before the flight, the cells in the pouches are supplied once more with supplements.

To apply the supplements a syringe is used. The pouch is already fixed in the clamp of the flight cassette bearing the ports for reagent supply.

Then, the pouches are inserted into the cassettes...

... and the clamps are screwed to the cassettes.

M2 macrophages generated from peripheral blood mononuclear cells using our M2-Macrophage Generation Medium DXF. As you can see, they are looking forward to experiencing weightlessness!

The map showing the forecasted weather and the area where we will be flying.

Dawn at Bordeaux-Mérignac airport. The A-300 ZERO-G is already being serviced.

Dr. Cora Thiel takes out one of the pouches with cells that have spent the night in an incubator rack and places it into the insulated transport box.

Our team on-site. From left to right: Christiane Raig (University of Zurich), Liliana Layer (PromoCell, Heidelberg), Prof. Oliver Ullrich (University of Zurich), Dr. Cora Thiel (University of Zurich), Claudia Philpot (DLR, Cologne), Hartwin Lier (KEK, Bad Schmiedeberg)

Inside the A-300 ZERO-G. The teams are working on their experiments

Dr. Cora Thiel, Prof. Oliver Ullrich and Liliana Layer just before starting their experiments.

Checking the inside of the experiment rack.

Here you can see three pouches of cells during the experiment. Through the ports on the bottom of each cassette reagents are being injected to the cells at given timepoints during one parabola.

And this is part of the electrical technics of the experimental rack on the rear side.

The pilots are preparing for the next parabola. They always announce the seconds left until the next pull up, injection into zero-g or pull out.

Zero-g is fun! But always be sure to hold onto something. Free floating is not allowed in the experiment area.

Prof. Ullrich enjoying to float underneath the ceiling.

What is upside down in zero-g?

Our expert in fuzzy logic illegally free floating.

Liliana free floating legally in the free floating area restricted by nets.

After the parabolic flight the samples are recovered and brought to the lab where the cells are further processed.

Videos:

Pictures and videos taken by the team of the 19th parabolic flight campaign unless indicated otherwise.

 


 

PromoCell Knowledge Base

The PromoCell online Knowledge Base upgrades our Technical Support and provides you with instant technical answers.

How do I use the Knowledge Base?

You can find the answers to detailed questions about our Products using 'Search for Information' (in our Support section). Type in your keyword and the Knowledge Base will show you the associated answers from our library of technical questions.

In the section Application Notes, you will find documents exceeding the information in our Product Manuals. For example, differentiation and staining protocols, all at your fingertips.

If you are interested in viewing published work by our customers, the section Reference Literature offers you access to literary citations of scientists that have used PromoCell Primary Cells or Media.

You can also search all categories with your keyword.
To try the new knowledge base, please click here.

Advantages of the Knowledge Base for you!

  • You save time, because accurate information is immediately available
  • You now have the choice to either contact our Technical Support team by phone or email, or you can immediately find your information online
  • You get more goal-oriented information

If you cannot find the answer to your question in our Knowledge Base, then please write us an email, or call our Technical Support Team with your query. We will respond to your question and integrate it in our Knowledge Base.

We also implemented an online Knowledge Base for our PromoKine products.
It provides you the answers to many questions about our cell biology products including:
Cell analysis and apoptosis, fluorescent labeling, cytokines & growth factors, cell transfection,
antibodies & ELISAs, and gene cloning.


PromoCells in Space

"It’s a milestone in our research. It was thrilling and relieving at the same time to do the count down and finally see the rocket launch.”, says Prof. Oliver Ullrich, space biologist from the Otto-von-Guericke-University in Magdeburg and the University of Zurich.

After 4 years of groundwork, the crewless research rocket TEXUS-49 finally took off. It was 6.01 am on the 29th of March 2011, at the Swedish rocket launch site ESRANGE near Kiruna, 150 km north of the Arctic Circle – and PromoCell’s Hematopoietic Stem Cells were on board.
TEXUS (a German acronym for technological experiments in weightlessness) number 49 set off to analyze why human cells display long-term sensitivity to weightlessness – this is one of the most important problems for manned space flights and it is the research project of Prof. Oliver Ullrich. Since the first crewed spaceflights occurred, it is known that during and after long periods in weightlessness, astronauts suffer from serious disorders of the immune system that manifest themselves in heavy infections.

Oliver Ullrich has already gained valuable insight into the signal transduction of cells of the immune system from eight parabolic flight campaigns. In March, this project was taken to the next step, and our PromoCell Technical Customer Service was right in the middle of the action.

During the 20 minute flight, PromoCell’s Hematopoietic Stem Cells were exposed to microgravity for approximately 6 minutes. Prof. Oliver Ullrich knows from experience: “The immune cells react within seconds of time to the suspension of gravity.”

After its flight at a height of up to 268 km, the TEXUS Payload landed with the Stem Cells in an unpopulated area of northern Sweden and was recovered by helicopter.

The setup with PromoCell’s Hematopoietic Stem Cells is now back in the laboratory in Zurich, where the experiments are being analyzed using modern technology.

At this point in time, one thing’s for sure: the story of “PromoCells in Space” will continue – further tests in weightlessness are planned!

 

 

TEXUS-49: Signal Transduction in Cells of the Immune System in Microgravity (SITI-1).

The Swedish rocket launch site ESRANGE near Kiruna, 150 km north of the Arctic Circle.

View over ESRANGE

Our PromoCell Technical Customer Service (TCS), Liliana Layer, braves the danger for science.

An Aurora borealis seen at ESRANGE space center.

One of the incubators at the ESRANGE lab.

PromoCell’s TCS at work.

Prof. Oliver Ullrich dreaming about the results.

PromoCell’s TCS right in the middle of the action (from left to right: Dipl. biol. Svantje Tauber, TCS Liliana Layer, Prof. Dr. Oliver Ullrich).

The rocket nosecone is being prepared.

Rocket technicians assembling the electronics.

PromoCell’s Hematopoietic Stem Cells are transferred to syringes, ...

... mounted into holders, ...

... and prepared for stacking...

... by special staff.

An experiment module.

The cells are contained within a temperature-controlled device powered by several packages of built-in batteries.

Last assemblies being made on the TEXUS-49.

The TEXUS-49 without motor.

The TEXUS-49 is brought to...

…the launch tower.

Dawn at ESRANGE.

Spectators of the take-off.

Launch of TEXUS-49 on March 29, 2011 (Photo: Adrian Mettauer).

Launch of TEXUS-49 on March 29, 2011 (Photo: Adrian Mettauer).

Launch of TEXUS-49 on March 29, 2011 (Photo: Adrian Mettauer).

Launch of TEXUS-49 on March 29, 2011 (Photo: Adrian Mettauer).

On board camera TEXUS-49: March 29, 2011.

On board camera: Lapland from space.

The Payload is recovered by helicopter...

... by members of the TEXUS-49 team.

PromoCell’s Hematopoietic Stem Cells after their recovery from the Payload.

The story of “PromoCells in Space” will continue – further tests on them in weightlessness are planned! See you soon!

All pictures were taken by members of the TEXUS-49 Team, except indicated otherwise.