Products

PromoCell - Mesenchymal Stem Cell Culture System

Complete portfolio of human MSC, growth and differentiation media



Overview

PromoCell’s human Mesenchymal Stem Cells (hMSC) are available as cryopreserved or proliferating cells isolated from bone marrow, adipose tissue, and umbilical cord matrix of individual donors. Our optimized MSC Growth Media are designed to robustly support the expansion of multipotent human MSC while retaining maximum multipotency. In addition, PromoCell’s MSC Differentia­tion Media allow for directed and efficient differentiation of human MSC into adipocytes, osteoblasts, chondro­cytes and neurons. Detailed step-by-step protocols are available in our Application Notes on our website.

Key Features

  • Guaranteed Growth Performance
    All of our MSC are guaranteed to grow with a doubling time of <30h for a minimum of 10 population doublings.

  • Differentiation Tested
    Each MSC lot is tested for differentiation into osteoblasts, adipocytes and chondrocytes in accordance with ISCT* guidelines.

  • ISCT* Marker Panel
    Each MSC lot is tested for CD73+/CD90+/CD105+ and CD14-/CD19-/CD34-/CD45-/HLA-DR-.

  • Higher Result Comparability
    Our newly designed MSC Growth Medium 2 with low-serum content (2% FCS) ensures low batch-to-batch variation.

  • Defined Medium
    DXF Medium available for defined and xeno-free MSC culture. 

*ISCT (International Society for Cellular Therapy) Cytotherapy (2006) Vol. 8, No. 4, 315-317

Product Name Size Catalog Number Information Prices
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM) 500,000 cryopreserved cells C-12974 click to select country
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT) 500,000 cryopreserved cells C-12977 click to select country
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC) 500,000 cryopreserved cells C-12971 click to select country
Mesenchymal Stem Cell Growth Medium 2 (Ready-to-use) 500 ml C-28009 click to select country
Mesenchymal Stem Cell Growth Medium DXF (Ready-to-use) 500 ml C-28019 click to select country


MSC Differentiation Media: Adipogenic, Chondrogenic, Osteogenic, Neurogenic See here

Application Notes – MSC Differentiation and Analysis

MSC Adipogenic Differentiation
MSC Chondrogenic Differentiation
MSC Osteogenic Differentiation
MSC Neurogenic Differentiation

Quality Control & Characterization

PromoCell’s human MSC are isolated in accordance to the highest ethical standards and extensively quality tested including marker characterization, growth performance, and in vitro differentiation. Each produced MSC lot is stringently tested for:

  • Cellular markers: positive for CD73, CD90, CD105, negative for CD14, CD19, CD34, CD45, HLA-DR expression
  • Differentiation potential into chondrogenic, apidogenic and osteogenic lineages.
  • Growth performance and morphology during initial 10 population doublings. 

Data and Publications

Figure 1. Flow cytometry analysis of PromoCell human primary mesenchymal stem cells isolated from bone marrow. Cryopreserved cells were tested after thawing and show a well-defined population positive for the markers CD73, CD90 and CD105 and negative for CD14 or CD11b, CD34, CD45, CD79α or CD19, and HLA-DR, in agreement with ISCT guidelines.

 

 

Figure 2. Growth performance of PromoCell human primary mesenchymal stem cells (MSCs) isolated from bone marrow cultured in PromoCell MSC Growth Medium DXF on fibronectin-coated tissue culture plastic. The MSCs used in this investigation all meet the International Society for Cellular Therapy (ISCT) criteria. The cumulative number of population doublings and doubling time is shown over the course of seven passages. A stable growth rate of under 30h/doubling can be observed even after prolonged in vitro culture for 32 population doublings over the course of 7 passages.

Figure 3. Differentiation of in vitro cultured PromoCell human mesenchymal stem cells (MSCs) into adipocytes (A), chondrocytes (B), and osteoblasts (C). According to the International Society for Cellular Therapy (ISCT) criteria, MSCs must differentiate into all three cell types independent of their origin.  (A) Lipid vesicle accumulation in adipocytes differentiated from human MSC derived from bone marrow (hMSC-BM) using the PromoCell MSC Adipogenic Differentiation Medium 2. The differentiated culture exhibits extensive intracellular lipid vacuole formation typical of mature adipocytes (100x magnification). (B) MSC spheroids after in vitro differentiation into cartilage using PromoCell MSC Chondrogenic Differentiation Medium (stained for aggrecans using Alcian Blue). Induced spheroids exhibit an intensely blue color indicative of cartilage extracellular matrix. (C) Alizarin Red S staining of extracellular calcium deposits in mineralized hMSC-BM -derived mature osteoblasts. Cells were cultured for 12 days in PromoCell MSC Osteogenic Differentiation Medium.

 

Publications:

For scientific references concerning our Mesenchymal Stem Cells and Media, please click here.