Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus Pectoralis Major) of adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin as well as endothelial cell specific von Willebrand factor (vWF).
New skeletal muscle cells originate from quiescent myoblasts, also called satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent myoblasts are activated by stimuli such as muscle damage. After activation, they start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.
SkMC are optimal for in vitro muscle studies. They proliferate very well in the mitogen-rich PromoCell Skeletal Muscle Cell Growth Medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using the PromoCell Skeletal Muscle Cell Differentiation Medium.
The cells have been tested: Sarcomeric myosin positive, Smooth muscle alpha-actin negative, Von Willebrand factor (vWF) negative
