Overview Human Primary Cells

Recommended Culture Media*	Product	Plating Density	Passage after Thawing	Marker*	Population Doublings
C-22070	Human Cardiac Myocytes (HCM)	10,000 - 15,000 cells per cm²	P2	Sarcomeric alpha-Actinin+ Slow muscle myosin+	> 15
C-27101	Human Chondrocytes (HCH)	10,000 - 20,000 cells per cm²	P2	Differentiation tested	> 10
C-22010 C-22011	Human Umbilical Vein Endothelial Cells (HUVEC)	5,000 - 10,000 cells per cm²	P1	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22010 C-22011	Human Umbilical Vein Endothelial Cells (HUVEC) pooled	5,000 - 10,000 cells per cm²	P1	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22010 C-22011	Human Umbilical Vein Endothelial Cells (HUVEC) pre-screened	5,000 - 10,000 cells per cm²	P1	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22010 C-22011	Human Umbilical Artery Endothelial Cells (HUAEC)	5,000 - 10,000 cells per cm²	P1	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Aortic Endothelial Cells (HAoEC)	5,000 - 10,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Coronary Artery Endothelial Cells (HCAEC)	5,000 - 10,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22010 C-22011	Human Pulmonary Artery Endothelial Cells (HPAEC)	5,000 - 10,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22010 C-22011	Human Saphenous Vein Endothelial Cells (HSaVEC)	5,000 - 10,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Dermal Microvascular Endothelial Cells (HDMEC) juvenile foreskin	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Dermal Microvascular Endothelial Cells (HDMEC) adult donor	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Dermal Microvascular Endothelial Cells (HDMEC) pre-screened	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020	Human Dermal Blood Endothelial Cells (HDBEC) juvenile foreskin	10,000 - 20,000 cells per cm²	P2	Podoplanin^- Prox-1^- CD31⁺	> 15
C-22020	Human Dermal Blood Endothelial Cells (HDBEC) adult donor	10,000 - 20,000 cells per cm²	P2	Podoplanin^- Prox-1^- CD31⁺	> 15
C-22020	Human Dermal Lymphatic Endothelial Cells (HDLEC) juvenile foreskin	10,000 - 20,000 cells per cm²	P2	Podoplanin^- Prox-1^- CD31⁺	> 15
C-22020	Human Dermal Lymphatic Endothelial Cells (HDLEC) adult donor	10,000 - 20,000 cells per cm²	P2	Podoplanin^- Prox-1^- CD31⁺	> 15
C-22020 C-22022	Human Bladder Microvascular Endothelial Cells (HBdMEC)	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Cardiac Microvascular Endothelial Cells (HCMEC)	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Pulmonary Microvascular Endothelial Cells (HPMEC)	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-22020 C-22022	Human Uterine Microvascular Endothelial Cells (HUtMEC)	10,000 - 20,000 cells per cm²	P2	vWF⁺ CD31⁺ Dil-Ac-LDL uptake⁺	> 15
C-21060	Human Nasal Epithelial Cells (HNEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-21060	Human Tracheal Epithelial Cells (HTEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-21060	Human Bronchial Epithelial Cells (HBEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-21070	Human Small Airway Epithelial Cells (HSAEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-21010	Human Mammary Epithelial Cells (HMEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-26001 C-26030	Human Renal Epithelial Cells (HREpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-26001 C-26030	Human Renal Cortical Epithelial Cells (HRCEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-26040	Human Placental Epithelial Cells (HPlEpC)	10,000 - 15,000 cells per cm²	P2	Cytokeratin⁺	> 5
C-23010	Normal Human Dermal Fibroblasts (NHDF) juvenile foreskin	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 15
C-23020	Normal Human Dermal Fibroblasts (NHDF) adult donor	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 15
C-23020	Human Pulmonary Fibroblasts (HPF)	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 15
C-23020	Human Aortic Adventitial Fibroblasts (HAoAF)	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 10
C-23025	Human Cardiac Fibroblasts (HCF)	3,500 - 7,000 cells per cm²	P2	CD90⁺ Smooth muscle α-actin^- Slow muscle myosin^-	> 15
C-23020	Human Uterine Fibroblasts (HUF)	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 15
C-23020	Human Villous Mesenchymal Fibroblasts (HVMF)	3,500 - 7,000 cells per cm²	P2	CD90⁺	> 15
C-26501	Human Follicle Dermal Papilla Cells (HFDPC)	5,000 - 10,000 cells per cm²	P2	Alkaline phosphatase⁺	> 10
C-25010 C-25020	Human Hepatocytes (HHpC)	150,000 cells per cm²	P0	ECOD⁺ 7-HCG/7-HCS⁺ Albumin⁺
C-20011	Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor	5,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-20011	Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled	5,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-20011	Normal Human Epidermal Keratinocytes (NHEK) adult, single donor	5,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-20011	Normal Human Epidermal Keratinocytes (NHEK) adult, pooled	5,000 cells per cm²	P2	Cytokeratin⁺	> 15
C-24010	Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin	5,000 - 10,000 cells per cm²	P2	Mel-5⁺	> 15
C-24300	Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M2 Medium	5,000 - 10,000 cells per cm²	P2	Mel-5⁺	> 15
C-24300	Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M2 Medium	5,000 - 10,000 cells per cm²	P2	Mel-5⁺	> 15
C-27001	Human Osteoblasts (HOB)	10,000 - 20,000 cells per cm²	P2	Alkaline phosphatase⁺ Mineralization tested	> 10
C-27410 C-27436 C-27438	Human White Preadipocytes (HWP) subcutaneous	5,000 cells per cm²	P2	Differentiation tested	> 10
C-27410 C-27436 C-27438	Human White Preadipocytes (HWP) visceral	5,000 cells per cm²	P2	Differentiation tested	> 10
C-23060 C-23061	Human Skeletal Muscle Cells (SkMC)	3,500 - 7,000 cells per cm²	P2	Sarcomeric Myosin⁺ Differentiation tested	> 15
C-22062	Human Aortic Smooth Muscle Cells (HAoSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Coronary Artery Smooth Muscle Cells (HCASMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Pulmonary Artery Smooth Muscle Cells (HPASMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Umbilical Artery Smooth Muscle Cells (HUASMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Tracheal Smooth Muscle Cells (HTSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Bronchial Smooth Muscle Cells (HBSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Bladder Smooth Muscle Cells (HBdSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Prostate Smooth Muscle Cells (HPrSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15
C-22062	Human Uterine Smooth Muscle Cells (HUtSMC)	7,500 - 10,000 cells per cm²	P2	Smooth muscle alpha-Actin⁺	> 15

* The media in this table are ready-to-use. For Media Kits see chapter Media for Primary Cells.
** CD 31, Prox-1 and Podoplanin Antibodies are available from PromoKine.