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Melanocytes

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs.
(Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF)  from the same donor are available on request.)

They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
PromoCell melanocytes are isolated using either the serum-free, PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium or the serum-free and PMA-free Melanocyte Growth Medium M2. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.

Figure: Structure of the epidermis with the location of a melanocyte.

Recommended media & reagents

Related products

References, Questions & Answers

Product Name Size Catalog Number Information Prices
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin 500,000 cryopreserved cells C-12400 click to select country
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin 500,000 proliferating cells C-12450 click to select country
NHEM.f Pellet 1x106 cells in RNAlater C-14040 click to select country
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M2 Medium 500,000 cryopreserved cells C-12402 click to select country
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M2 Medium 500,000 proliferating cells C-12452 click to select country
NHEM.f M2 Pellet 1x106 cells in RNAlater C-14042 click to select country
Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M2 Medium 500,000 cryopreserved cells C-12403 click to select country
Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M2 Medium 500,000 proliferating cells C-12453 click to select country
NHEM M2 adult Pellet 1x106 cells in RNAlater C-14043 click to select country