Human Uterine Microvascular Endothelial Cells (HUtMEC)

Primary Human Uterine Microvascular Endothelial Cells isolated from the myometrium from a single donor. Donors not pre-treated with hormones.

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Selected product Human Uterine Microvascular Endothelial Cells (HUtMEC)

cryopreserved 500,000 cells

proliferating 500,000 cells

cell pellet in RNAlater 1,000,000 cells

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Primary Human Uterine Microvascular Endothelial Cells (HUtMEC) are isolated from the myometrium (middle layer of the uterine wall) from a single donor who is not pre-treated with hormones. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.

During pregnancy HUtMEC propagate intensely and change their phenotype and expression pattern in response to sex steroids. This includes up-regulation of HUtMEC nitric oxide synthase activity, modulation of adhesion molecule expression, and an increase in endothelial cell survival and angiogenic activity. HUtMEC are involved in medical conditions like uterine fibroma or adeno-mysis.

NEW:

Available formats:

  • Cryopreserved: Cryogenic vial containing 500.000 viable cells.
  • Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density 10,000 – 20,000 cells per cm2
Passage After Thawing P2
Tested Markers CD31 positive, Dil-Ac-LDL uptake positive
Guaranteed Population Doubling > 15

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