Macrophage Generation Medium DXF

M1-Macrophage Generation Medium DXF
M2-Macrophage Generation Medium DXF
Monocyte Attachment Medium

The PromoCell Macrophage Generation Medium was developed for easy and efficient generation of M1- or M2-polarized macrophages from freshly isolated peripheral blood monocytes. A detailed step-by-step protocol is available also, which allows optional user-customized macrophage activation.

In combination with the Monocyte Attachment Medium, freshly isolated peripheral blood mononuclear cells can also be directly used as a starting material.

The PromoCell M1-Macrophage Generation Medium DXF allows for the generation of M1-polarized macrophages, whereas the M2-Macrophage Generation Medium DXF produces M2-polarized macrophages. The defined and xeno-free media formulations are free of all animal derived components and substances of human origin with Human Serum Albumin as the only exception.

The Macrophage Base Medium does not include cytokines and must therefore be supplemented according to the user’s individual needs.

The Monocyte Attachment Medium allows for the efficient adherence of fresh monocytes as well as for adherence selection of monocytes from freshly isolated Mononuclear Cells (MNC) while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes prior to macrophage differentiation is not necessary.

Figure: Schematic overview on the user-customizable M1-/M2-Macrophage differentiation process using the PromoCell Macrophage Generation Media.

Note: See our Instruction Manual and Application Note for details.

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References, Questions & Answers

Product Name Size Catalog Number Information Prices
M1-Macrophage Generation Medium DXF (Ready-to-use) 250 ml C-28055 click to select country
M2-Macrophage Generation Medium DXF (Ready-to-use) 250 ml C-28056 click to select country
Macrophage Base Medium DXF 250 ml C-28057 click to select country
Monocyte Attachment Medium (Ready-to-use) 250 ml C-28051 click to select country

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