Products

Hepatocyte Medium

Hepatocyte Growth Medium
Hepatocyte Maintenance Medium

The PromoCell Hepatocyte Growth Medium is a serum-free medium developed for the cultivation of human hepatocytes. It is supplemented with well chosen growth factors and hormones and suited for long-term cultivation.

In situations where the media compounds of the Hepatocyte Growth Medium interfere with certain experimental setups, we recommend using the PromoCell Hepatocyte Maintenance Medium. This medium is developed for short-term cultivation and contains fewer supplements.

Note: For culturing Hepatocytes, Collagen type I coated culture vessels are necessary. Under standard in vitro cell culture conditions, mature human hepatocytes usually do not survive for longer than 10-14 days and do not proliferate.

The media are optimized for primary human hepatocytes but can also be used for porcine hepatocytes.

Final supplement concentrations
(after addition to the medium)
Hepatocyte Growth MediumHepatocyte Maintenance Medium
Epidermal Growth Factor
(recombinant human)
10 ng / ml-
Insulin (recombinant human)5 μg / ml6 ng / ml
Hydrocortisone0.5 μg / ml-
Transferrin, holo (human)10 μg / ml-
Ascorbic Acid250 μg / ml-
Bovine Serum Albumin-Fatty Acid Free
(BSA-FAF)
3.75 mg / ml-
Dexamethasone-400 ng / ml

References, Questions & Answers

Product Name Size Catalog Number Information Prices
Hepatocyte Growth Medium (Ready-to-use) 500 ml C-25010 click to select country
Hepatocyte Growth Medium Kit 500 ml C-25110 click to select country
Hepatocyte Basal Medium 500 ml C-25210 click to select country
Hepatocyte Basal Medium, phenol red-free 500 ml C-25215 click to select country
Hepatocyte Growth Medium SupplementMix 1 Mix for 500 ml C-39642 click to select country
Hepatocyte Growth Medium SupplementPack 1 Pack for 500 ml C-39640 click to select country
Hepatocyte Maintenance Medium (Ready-to-use) 500 ml C-25020 click to select country
Hepatocyte Maintenance Medium Kit 500 ml C-25120 click to select country
Hepatocyte Maintenance Medium SupplementMix 1 Mix for 500 ml C-39652 click to select country
Hepatocyte Maintenance Medium SupplementPack 1 Pack for 500 ml C-39650 click to select country