Products

Endothelial Cell Medium

Endothelial Cell Growth Medium
Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium and Endothelial Cell Growth Medium 2 are low-serum (2% V/V) media optimized for the cultivation of endothelial cells from large blood vessels.

Endothelial Cell Growth Medium 2 lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on cell metabolism, it may also interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.

The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.

To view a list of references where this media was used with other species/cell types please click here.

Final supplement concentrations
(after addition to the medium)
Endothelial Cell Growth MediumEndothelial Cell Growth Medium 2
Fetal Calf Serum0.02 ml / ml0.02 ml / ml
Endothelial Cell Growth Supplement0.004 ml / ml-
Epidermal Growth Factor
(recombinant human)
0.1 ng / ml5 ng / ml
Basic Fibroblast Growth Factor
(recombinant human)
1 ng / ml10 ng / ml
Insulin-like Growth Factor (Long R3 IGF)-20 ng / ml
Vascular Endothelial Growth Factor 165
(recombinant human)
-0.5 ng / ml
Ascorbic Acid-1 μg / ml
Heparin90 μg / ml22.5 μg / ml
Hydrocortisone1 μg / ml0.2 μg / ml

References, Questions & Answers

Product Name Size Catalog Number Information Prices
Endothelial Cell Growth Medium (Ready-to-use) 500 ml C-22010 click to select country
Endothelial Cell Basal Medium 500 ml C-22210 click to select country
Endothelial Cell Basal Medium, phenol red-free 500 ml C-22215 click to select country
Endothelial Cell Growth Medium SupplementMix 1 Mix for 500 ml C-39215 click to select country
Endothelial Cell Growth Medium Kit 500 ml C-22110 click to select country
Endothelial Cell Growth Medium SupplementPack 1 Pack for 500 ml C-39210 click to select country
Endothelial Cell Growth Medium 2 (Ready-to-use) 500 ml C-22011 click to select country
Endothelial Cell Growth Medium 2 Kit 500 ml C-22111 click to select country
Endothelial Cell Basal Medium 2 500 ml C-22211 click to select country
Endothelial Cell Basal Medium 2, phenol red-free 500 ml C-22216 click to select country
Endothelial Cell Growth Medium 2 SupplementMix 1 Mix for 500 ml C-39216 click to select country
Endothelial Cell Growth Medium 2 SupplementPack 1 Pack for 500 ml C-39211 click to select country