Endothelial Cell Media
Endothelial Cell Growth Medium
Endothelial Cell Growth Medium 2
Endothelial Cell Growth Medium and Endothelial Cell Growth Medium 2 are low-serum (2% V/V) media optimized for the cultivation of endothelial cells from large blood vessels.
Endothelial Cell Growth Medium 2 lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on cell metabolism, it may also interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.
The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.
| Final supplement concentrations (after addition to the medium) | Endothelial Cell Growth Medium | Endothelial Cell Growth Medium 2 |
|---|---|---|
| Fetal Calf Serum | 0.02 ml / ml | 0.02 ml / ml |
| Endothelial Cell Growth Supplement | 0.004 ml / ml | - |
| Epidermial Growth Factor (recombinant human) | 0.1 ng / ml | 5 ng / ml |
| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml | 10 ng / ml |
| Insulin-like Growth Factor (Long R3 IGF) | - | 20 ng / ml |
| Vascular Endothelial Growth Factor 165 (recombinant human) | - | 0.5 ng / ml |
| Ascorbic Acid | - | 1 μg / ml |
| Heparin | 90 μg / ml | 22.5 μg / ml |
| Hydrocortisone | 1 μg / ml | 0.2 μg / ml |
