Bioluminescent Cell Viability Kit I (ATP)
This kit utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell viability in mammalian cells.
The Promokine Bioluminescent Cell Viability Kit I employs bioluminescence to measure the ATP level as a marker for cell viability in mammalian cells. It utilizes the enzyme luciferase to catalyze the ATP-dependent, light-producing oxidation of luciferin. This light formation can be measured using a luminometer or beta counter.
The assay can be performed directly in culture plates requiring no harvest, washing, or sample preparation and can be fully automated for high throughput (10 seconds/sample). It is highly sensitive, detecting 10-100 mammalian cells/well, and it also offers new applications besides cell viability testing, e.g., detecting low level bacterial contamination in blood, milk, urine, soil, and sludge.