Anoikis is a form of cellular apoptosis induced upon detachment of cells from the surrounding extracellular matrix (ECM) and loss of normal cell-matrix interactions.
Anoikis is a hallmark of cancer. Cancer cells acquire anoikis resistance that is essential for successful metastasis and they exploit different strategies to overcome anoikis. Oxidative stress and hypoxia favor cell escaping from anoikis.
PromoCell provides an easy to use, sensitive assay to measure anoikis in cells and screen for compounds that influence/induce anoikis. The kit includes all required reagents and a proprietary coated chamber that mimics anoikis conditions, and results are obtained using a fluorescence plate reader.