Annexin V-Cy3 Reagent

Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy.

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Annexin V-Cy3 Reagent 1,000 assays

Annexin V-Cy3 Reagent 200 assays

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During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-Cy3 conjugate contains the bright red fluorescent probe that can be used for detection of apoptosis by fluorescence microscopy with a rhodamine filter or by flow cytometry. Cy3 yields red fluorescence with a λmax emission of 570 nm.