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Human primary cells are used by researchers worldwide as a physiologically relevant cell culture model to simulate biological processes, assay toxicity, as well as for carrying out pharmacological and genetic studies. PromoCell human primary cells and media portfolio offers ready-to-use solutions for culturing human primary cells from various adult tissues, stem and blood cells, and cancer cells.

Under our PromoKine brand, we offer a wide range of tools for assaying cell metabolism and viability, apoptosis research, cell transfection, fluorescent labeling, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

3D Cell Culture

In their natural physical environment, cells that form tissues and organs do not exist as single cell entities but are surrounded by other cells and are part of larger multicellular organisms. They are embedded within a complex non-cellular structure known as the extracellular matrix (ECM) which forms an interface between the cells and their adjacent stroma – anchoring and agglutinating the cells in a three-dimensional (3D) formation.

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Apoptosis

Apoptosis, or programmed cell death, is a sophisticated process that involves an intricate series of biochemical events. Specific cell death signals are transduced by different biomolecules such as caspases initiating an apoptotic cascade and finally causing multiple events such as degradation of cellular proteins and chromosomal DNA, mitochondrial disruption or changes in the plasma membrane integrity.

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Cell Disease Models

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Cell Model Systems

In vitro Cell Model Systems have become increasingly important for studying different cellular processes as well as creating and refining cellular models for the implementation of innovative therapeutic solutions (e.g. in cancer research).

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Cell Transfection

The ability to introduce foreign nucleic acids into eukaryotic cells has become a powerful tool in studying and controlling gene expression. Cloned genes can be transfected into cells for biochemical characterization, mutational analyses, investigating gene regulatory elements, and producing specific proteins. Cell transfection promotes the rapid advancement of our knowledge of genetic regulation and protein function.

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Cell Viability & Cell Proliferation

PromoCell offers six types of sensitive, convenient cell viability kits: The Fluorometric Cell Viability Kits I and II, the Colorimetric Cell Viability Kits I, II III and IV and the Bioluminescent Cell Viability Kits I and II. Since all kits are designed for use with various multiwell formats, they are also suited for automation.

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Dermal Cell Culture

The in vitro culture of human skin cells is an essential research tool widely used for assaying dermal toxicity, pharmacological testing, and tissue repair. We offer a comprehensive portfolio of primary human dermal cells including keratinocytes, melanocytes, fibroblasts and hair follicle cells to support these research applications.

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Endothelial Cell Culture

The in vitro culture of primary human endothelial cells is a widely established model system used to investigate several cardiovascular diseases and cancer research applications. Our comprehensive endothelial cell culture portfolio consists of 12 different types of large vessel and microvascular human endothelial cells with optimized growth media for each cell type.

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Fluorescent Labeling

The availability of new fluorophores has dramatically changed the possibilities for the sensitive detection of biomolecules and the analysis of their interactions. Improved fluorescent dyes are now enabling previously impossible studies of cellular structures and cellular processes. PromoCell provides a wide range of high-quality fluorophores for fluorescent labeling of diverse biomolecules: protein labeling, antibody labeling, nucleic acid labeling (DNA labeling, RNA labeling), as well as complete labeling kits and ready-to-use fluorescent conjugates.

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HLA-Typed Human Primary Cells

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Macrophage Cell Culture

The in vitro generation of monocyte-derived macrophages allows to investigate their role in numerous research applications. PromoCell provides a complete macrophage cell culture portfolio including macrophage generation media, cryopreserved human macrophages, cytokines and cell biology tools for subsequent analysis.

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Mesenchymal Stem Cell Culture

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages.

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Microbial Detection, Elimination & Prevention

Cell culture contamination is a commonly occurring problem that affects many of the cell’s biochemical and immunological properties, and it leads to unsatisfactory and unreliable results. Therefore, regularly testing cell cultures for contamination with mycoplasmas or bacteria is essential. PromoCell provides highly sensitive and user-friendly test kits for this purpose as well as products for the effective elimination and prevention of mycoplasma and eubacteria contamination.

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Myocyte Cell Culture

PromoCell’s myocyte cell culture portfolio features human striated muscle cells isolated from the adult heart and skeletal muscle tissue. Due to their ability to proliferate in vitro, our cardiac myocytes are widely used for assaying cardiotoxicity as well as in other pharmacological studies. Human skeletal muscle cells can be induced to differentiate into myotubes and are commonly used for investigating tissue repair, neuromuscular diseases, and pharmacological testing.

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Pulmonary Cell Culture

The human lung consists of several different cell types with highly specialized functions. Our comprehensive portfolio of primary human pulmonary cell types allows to model the characteristics of each cell type to investigate their role in developmental biological processes, pharmacological studies, and disease modeling.

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Renal Cell Culture

Our renal cell culture portfolio features primary human epithelial cells isolated from the adult kidney of individual donors. Reliable cell culture performance is ensured by our low-serum Renal Epithelial Cell Growth Medium 2.

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Smooth Muscle Cell Culture

Smooth muscle cells, which form non-striated muscles, are structurally and functionally different from skeletal and cardiac muscle cells. They are located in the walls of blood vessels and hollow organs like the bladder, the uterus, and the gastrointestinal tract and are responsible for involuntary movements like peristaltic contractions, which propel food along the gastrointestinal tract.

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